Changyu Bio supplies these PCR and qPCR reagents in-house for molecular biology research. The family covers dye-based qPCR (CYPC33; ROX is sold separately as CYPC38), FastLong / FastHiFi / LongHiFi endpoint mixes, one-step RT-PCR (CYPC34), and first-strand cDNA (CYPC1802). All CYPC catalogs ship on ice packs and store at −20°C. Research Use Only.
Available product families
2×SYBR Green qPCR Mix
Cat# CYPC33 — Dye-based SYBR Green qPCR; ROX not included (add CYPC38 if your cycler needs it). Research Use Only.
FastLong PCR MasterMix
Cat# CYPC80 — FastLong mix, about 5–10 s/kb up to ~10 kb; red dye; polyA ends for TA cloning. Research Use Only.
FastHiFi PCR Master Mix
Cat# CYPC82 — Engineered Pfu FastHiFi; blunt ends; typically ≤3 kb on complex genomes. Research Use Only.
LongHiFi PCR Master Mix
Cat# CYPC84 — LongHiFi; typically <10 kb complex / <15 kb simple templates; blunt ends. Research Use Only.
TRUEscript One Step RT-PCR Kit
Cat# CYPC34 — One-tube RT-PCR with gene-specific primers (not oligo(dT)/random). Research Use Only.
TRUEscript 1st Strand cDNA Synthesis Kit
Cat# CYPC1802 — First-strand cDNA up to 12 kb; omit gDNA Remover if you will clone. Research Use Only.
Brand-name PCR and qPCR reagents we also quote
If your protocol or tender names a specific brand, send the catalog number and we quote it together with the mixes above.
| Use | Bio-Rad | Thermo Fisher |
|---|---|---|
| SYBR Green qPCR master mix | iTaq Universal SYBR Green Supermix – 1725121 | PowerUp SYBR Green Master Mix (5 mL) – A25742 |
| cDNA synthesis | iScript cDNA Synthesis Kit – 1708891 | High-Capacity cDNA Reverse Transcription Kit – 4368814 |
| End-point PCR master mix | — | DreamTaq Green PCR Master Mix 2X (direct gel loading) – K1081 |
| One-step RT-qPCR (SYBR) | iTaq Universal SYBR Green One-Step Kit – 1725150 | Power SYBR Green RNA-to-CT 1-Step Kit – 4389986 |
For teaching labs: a 2× ready-to-use mix keeps pipetting steps few and repeatable across student groups. Tell us the number of classes and groups and we suggest reaction quantities. Instruments and plastics: Bio-Rad thermal cyclers, CFX qPCR and electrophoresis, PCR plates, strips, tips and tubes.
Brand names identify catalog items only. Changyu Bio is not an authorized distributor of these brands. All products are for Research Use Only.
ROX Reference Dye
Cat# CYPC38 — 100× ROX; ABI high vs low concentration; not needed on Roche/Bio-Rad/Eppendorf. Research Use Only.
Typical research applications
- Dye-based gene-expression qPCR with CYPC33 (add CYPC38 only if the cycler needs ROX)
- Endpoint PCR with polyA ends for TA cloning (CYPC80 FastLong)
- High-fidelity blunt PCR on short complex templates (CYPC82, typically ≤3 kb)
- Longer genomic amplicons with LongHiFi (CYPC84)
- One-tube RT-PCR with gene-specific primers (CYPC34 — not oligo(dT) or random primers)
- First-strand cDNA up to 12 kb (CYPC1802)
How these mixes differ
- Shipping — every CYPC catalog ships on ice packs. Store at −20°C on arrival; protect CYPC33 from light.
- ROX is a separate reagent — CYPC33 does not include ROX. CYPC38 is 100×; ABI instruments use high or low concentration; Roche, Bio-Rad and Eppendorf cyclers usually skip it.
- Ends decide the cloning kit — FastLong (CYPC80) leaves polyA ends for pTOPO-TA. FastHiFi (CYPC82) and LongHiFi (CYPC84) leave blunt ends for pTOPO-Blunt.
- One-step vs two-step RNA — CYPC34 is one-tube RT-PCR with gene-specific primers. CYPC1802 makes first-strand cDNA; omit gDNA Remover if you will clone the product.
Frequently asked questions
- How are the mixes shipped? Ice packs for every CYPC catalog. We do not use dry ice unless you ask and we confirm. Store at the temperature on each product page on arrival.
- Does 2×SYBR Green qPCR Mix include ROX? No. Add CYPC38 only if your instrument protocol requires it.
- FastHiFi vs LongHiFi vs FastLong? FastHiFi (CYPC82) is engineered Pfu, blunt, typically ≤3 kb on complex genomes. LongHiFi (CYPC84) is for longer templates, also blunt. FastLong (CYPC80) is about 5–10 s/kb up to ~10 kb, red dye, polyA ends.
- What pack sizes can I quote? Use the sizes on each product page (CYPC33 is 50 or 200 reactions). There is no list price on this site — request a quote.
- Are these products for clinical use? No. Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical applications.
Request a quotation · Email market@changyubio.com · WhatsApp +86 18665577773
All products are for Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use.
Looking for an alternative to your current kit?
Start with the relevant product questions below. Match the sample, method and downstream requirements before switching; protocol settings and kit components are not assumed to be interchangeable.
- Is CYPC1802 an alternative to RevertAid K1622 for first-strand cDNA synthesis?
- Can CYPC33 be considered instead of a SYBR Green qPCR mix from a major brand?
- Is CYPC34 an option when switching from a commercial one-step RT-PCR kit?
- Can CYPC38 be used when a qPCR workflow calls for a ROX passive reference?
- Can CYPC80 be considered instead of a routine Taq-based PCR master mix?
- Is CYPC82 an alternative to evaluate for a Q5 or Phusion high-fidelity PCR workflow?
- Can CYPC84 be considered for a long-amplicon high-fidelity workflow?
More catalog products
- CYPC64 THERMOscript RT Kit (+gDNA Eraser)
- CYPC58 TRUEscript RT MasterMix (for Real Time PCR)
- CYPC68 THERMOscript RT MasterMix (For Real Time PCR)
- CYPC45 THERMOscript One Step RT-PCR Kit
- CYPC85 TRUEscript One Step qRT-PCR Probe Kit
- CYPC41 TRUEscript One Step qRT-PCR SYBR Green Kit
- CYPC42 THERMOscript One Step qRT-PCR SYBR Green Kit
- CYPC65 miRNA cDNA Kit (Stem-Loop)
- CYPC90 2x miRNA SYBR Green qPCR Mix (Universal ROX)
- CYPC70 TRUEscript RT MasterMix (OneStep gDNA Removal)
- CYPC88 THERMOscript RT MasterMix (OneStep gDNA Removal)
- CYPC17 TRUEscript H Minus M-MuLV Reverse Transcriptase
- CYPC43 THERMOscript H Minus M-MuLV Reverse Transcriptase
- CYPC18 TRUEscript 1st Strand cDNA Synthesis Kit (OneStep gDNA Removal)
- CYPC44 THERMOscript 1st Strand cDNA Synthesis Kit (OneStep gDNA Removal)
- CYPC54 TRUEscript RT Kit (+gDNA Eraser)
- CYPC46 THERMOscript SYBR Green qRT-PCR Kit (Two-Step)
- CYPC40 20x SYBR Green I (For Real-Time PCR)
- CYPC11 2x Pfu PCR MasterMix
- CYPC03 SuperPfu DNA Polymerase
- CYPC57 2x KOD PCR MasterMix
- CYPC56 KOD DNA Polymerase
- CYPC86 2x Rapid PCR MasterMix
- CYPC15 2x Long Taq PCR MasterMix
- CYPC08 Long Taq DNA Polymerase
- CYPC13 2x HotMaster Taq PCR MasterMix
- CYPC05 HotMaster Taq DNA Polymerase (Hot-Start)
- CYPC37 2x Taq Plus PCR MasterMix
- CYPC07 Taq Plus DNA Polymerase
- CYPC09 2x Taq PCR MasterMix
- CYPC01 Taq DNA Polymerase (Buffer With Mg2+)
- CYPC50 2x Taq PCR MasterMix for PAGE (With Dye)
- CYPC23 dNTP Mixture 2.5 mM Each
- CYPC24 dNTP Mixture 10 mM Each
- CYPC51 SuperPure dNTP Mixture 2.5 mM Each
- CYPC52 SuperPure dNTP Mixture 10 mM Each
- CYPC25 dATP/dTTP/dGTP/dCTP 100 mM Individual Solutions
- CYPC22 PCR Enhancer
- CYPC60 2x SYBR Green qPCR Mix (Low ROX)
- CYPC61 2x SYBR Green qPCR Mix (High ROX)
- CYPC59 2x SYBR Green qPCR Mix (With 100x ROX)
- CYPC62 2x Dual SYBR Green qPCR Mix (Universal ROX)
- CYPC91 2x Dual SYBR qPCR Mix (Blue Dye)
- CYPC39 2x Real Time PCR Mix (Probe)
- CYPC63 Enhanced miRNA RT/qPCR Detection Kit (All In One)
- CYPC48 Enhanced miRNA First-Strand Synthesis Kit
- CYPC49 Enhanced miRNA qPCR Detection Kit
- CYPC36 TRUEscript SYBR Green qRT-PCR Kit (Two-Step)
- CYPC92 Lysis Buffer for PCR
- CYPC55 DirectAmp Mouse Tail PCR Kit
- CYPC31 DirectAmp Plant PCR Kit
- CYPC53 DirectAmp Animal Tissue PCR Kit
- CYDP01 DirectAmp Avian ID Kit
- CYPC89 DirectAmp Yeast Colony PCR Kit