CYPC60 is a 2x SYBR Green I real-time PCR master mix that contains hot-start HotMaster Taq DNA polymerase, dNTPs, a stabilizer, optimized buffer, BSA and SYBR Green I premixed as a 2x reagent. It is used by adding template, primers and water.
Catalog no. CYPC60 · For research use only
Key features
- Premixed hot-start HotMaster Taq DNA polymerase, dNTPs, stabilizer, buffer, BSA and SYBR Green I
- Temperature-controlled hot start limits nonspecific amplification
- Low-concentration ROX included, for instruments that need low ROX
- Gives a standard curve over a wide quantitative range for target gene quantification
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC6001 | 2x SYBR Green qPCR Mix (Low ROX) | 125 reactions (20 μL) |
| CYPC6002 | 2x SYBR Green qPCR Mix (Low ROX) | 500 reactions (20 μL) |
Specifications
| Format | 2x premix, SYBR Green I detection |
|---|---|
| Enzyme | HotMaster Taq DNA polymerase (hot start) |
| ROX | Low ROX |
| Storage | -20 °C, protected from light, at least 12 months |
| Working storage | 4 °C short term; avoid repeated freeze-thaw |
| Use | Research use only |
Applications
- SYBR Green I real-time PCR quantification of target genes
- Gene expression analysis by qPCR
- Quantitative detection with a standard curve
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
2x SYBR Green qPCR Mix (Low ROX) FAQ
Does it contain ROX?
Yes, a low concentration. It suits ABI 7500, 7500 Fast, Q6, ViiA 7, QuantStudio 6/7 Flex, Stratagene MX4000, MX3005, MX3000, Corbett Rotor-Gene 3000 and other low-ROX instruments.
How is it stored?
At -20 °C protected from light for at least 12 months. Thaw and mix fully before use; short-term use can be at 4 °C. Avoid repeated freeze-thaw.
How does the hot start work?
HotMaster Taq DNA polymerase blocks the substrate-binding site with an inhibitory ligand in a temperature-dependent way: below 40 °C an inactive enzyme-inhibitor complex forms, and at the primer annealing temperature the equilibrium shifts toward the template-primer complex.
How do I order CYPC60?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.