Changyu pTOPO kits clone PCR products for research use: TA (CYCV14) or TA Simple (CYCV15) for Taq A-overhangs; Blunt (CYCV16) or Blunt Simple (CYCV17) for Pfu/KOD/Phusion products. DNA must not be 5′-phosphorylated. Kits ship on ice packs; competent cells are not in the box. Research Use Only.
Choose your pTOPO kit
| Kit | Cat# | Insert ends | For PCR products from | Reactions per kit | Manual / quote |
|---|---|---|---|---|---|
| pTOPO-TA | CYCV14 | A-overhangs | Taq-type polymerases (e.g., FastLong) | CYCV1401: 20 × 10 µl or 40 × 5 µl CYCV1402: 80 × 10 µl or 160 × 5 µl | Manual (PDF) · Quote CYCV1401 · Quote CYCV1402 |
| pTOPO-TA Simple | CYCV15 | A-overhangs | Taq-type polymerases; no flanking restriction sites | CYCV1501: 20 × 10 µl or 40 × 5 µl CYCV1502: 80 × 10 µl or 160 × 5 µl | Manual (PDF) · Quote CYCV1501 · Quote CYCV1502 |
| pTOPO-Blunt | CYCV16 | Blunt | Proofreading polymerases (e.g., FastHiFi, LongHiFi) — no A-tailing | CYCV1601: 20 × 10 µl or 40 × 5 µl CYCV1602: 80 × 10 µl or 160 × 5 µl | Manual (PDF) · Quote CYCV1601 · Quote CYCV1602 |
| pTOPO-Blunt Simple | CYCV17 | Blunt | Proofreading polymerases; no flanking restriction sites | CYCV1701: 20 × 10 µl or 40 × 5 µl CYCV1702: 80 × 10 µl or 160 × 5 µl | Manual (PDF) · Quote CYCV1701 · Quote CYCV1702 |
| pTOPO-TA/Blunt | CYCV21 | A-overhangs or blunt | Any PCR product, Taq or high-fidelity | CYCV2101: 20 × 10 µl or 40 × 5 µl CYCV2102: 80 × 10 µl or 160 × 5 µl | Manual (PDF) · Quote CYCV2101 · Quote CYCV2102 |
| pTOPO-TA/Blunt Simple | CYCV22 | A-overhangs or blunt | Any PCR product; no flanking restriction sites | CYCV2201: 20 × 10 µl or 40 × 5 µl CYCV2202: 80 × 10 µl or 160 × 5 µl | Manual (PDF) · Quote CYCV2201 · Quote CYCV2202 |
All six kits: 5-minute reaction at 25–37°C (set up at room temperature, not on ice), recommended DNA amounts given for 100 bp–5 kb inserts (stated design limit 10 kb; long inserts may need 10–15 min at 37°C), zero-background selection (no blue-white screening), ampicillin resistance, and no competent cells or ligase included (manual). We have no head-to-head data against Thermo Fisher TOPO kits; choose by insert-end type and vector features, and test with your own insert.
Replacing a Thermo Fisher / Invitrogen TOPO kit?
| If you use now | Changyu alternative | Check before switching |
|---|---|---|
| Zero Blunt TOPO PCR Cloning Kit (e.g. 450245) | CYCV16 or CYCV17 | Our vector is ampicillin-resistant: plate on LB + 100 µg/ml ampicillin, not kanamycin or Zeocin. Sequence with M13F/M13R (not M13(-47)/(-48)). |
| TOPO TA Cloning Kit | CYCV14 or CYCV15 | Zero-background selection replaces blue-white screening, so no X-gal/IPTG plates are needed. |
| Both TA and blunt products in one lab | CYCV21 or CYCV22 | One vector accepts A-overhang and blunt PCR products. |
Competent cells are not included in any of these kits; use your usual chemically competent E. coli. We have no head-to-head data against Thermo Fisher kits, so we suggest testing one kit with your own insert first. Thermo Fisher, Invitrogen and Zeocin are trademarks of their owners; Changyu kits are independent products.
International orders (Latin America, Europe, Africa, Asia): single-unit orders are accepted. Quotes and invoices are in USD, payment by company bank transfer. Kits ship with ice packs and freight is quoted to your city at actual cost; other reagents can be added to the same shipment to share one freight charge. Tell us your institution and whether you import through an agent or an import exemption, and we will prepare the documents to match.
Available product families
pTOPO-TA Cloning Kit
Cat# CYCV14 — TA cloning of Taq products; DNA must not be 5′-phosphorylated. Research Use Only.
pTOPO-TA Simple Cloning Kit
Cat# CYCV15 — TA Simple chemistry; Taq A-overhang fragments; do not 5′-phosphorylate. Research Use Only.
pTOPO-Blunt Cloning Kit
Cat# CYCV16 — Blunt cloning of Pfu/KOD/Phusion products; do not 5′-phosphorylate. Research Use Only.
pTOPO-Blunt Simple Cloning Kit
Cat# CYCV17 — Blunt Simple chemistry; same blunt-end rule as CYCV16. Research Use Only.
Typical research applications
- Cloning Taq PCR products with 3′-A overhangs (CYCV14 / CYCV15)
- Cloning blunt products from Pfu, KOD or Phusion-type polymerases (CYCV16 / CYCV17)
- Recommended DNA amounts are given for inserts of about 100 bp to 5 kb; the stated design limit is 10 kb
- Preparing cloned templates for sequencing
TA, Blunt, or Simple?
- Match the polymerase — TA kits (CYCV14, CYCV15) for Taq A-overhang fragments. Blunt kits (CYCV16, CYCV17) for Pfu/KOD/Phusion products.
- Standard vs Simple — Standard vectors (CYCV14, CYCV16) have common restriction sites flanking the cloning site so you can cut the insert out. Simple vectors (CYCV15, CYCV17) omit those flanking sites to avoid redundant restriction sites in the insert.
- Do not 5′-phosphorylate the DNA — all six kits (CYCV14–17, CYCV21, CYCV22) require free 5′-OH ends. Competent cells are not in the box (vector, 1000 bp control, enhancer only).
- Shipping — ice packs; store at −20°C (12 months in the manuals).
International ordering
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment; transit time and cold-chain packing for your destination are confirmed on the quotation.
- Shipped with ice packs; store at −20 °C on arrival.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation — tell us the quantity and destination city.
Frequently asked questions
- TA or Blunt? If the amplicon came from Taq (including FastLong CYPC80), use CYCV14 or CYCV15. If it came from a high-fidelity blunt polymerase (FastHiFi CYPC82, LongHiFi CYPC84), use CYCV16 or CYCV17.
- What does Simple mean? The Simple vectors do not carry common restriction sites next to the cloning site. Use them when those sites would collide with sites inside your insert.
- Do the kits include competent cells? No. Listed contents are the pTOPO vector, a 1000 bp control insert, and enhancer. Ask if you also need cells.
- How are kits shipped? Ice packs for every CYCV catalog. Store at −20°C on arrival.
- Are these kits for clinical use? No. Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use.
Request a quotation · Email market@changyubio.com · WhatsApp +86 18665577773
All products are for Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use.
Looking for an alternative to your current kit?
Start with the relevant product questions below. Match the sample, method and downstream requirements before switching; protocol settings and kit components are not assumed to be interchangeable.
More catalog products
- CYCV2001 pTOPO-ENTR/D Directional Cloning Kit
- CYCV19 One Step Seamless Cloning Kit (Single Fragment)
- CYCV12 Seamless Assembly and Cloning Kit
- CYCV08 pZERO-Blunt Cloning Kit
- CYCV13 pZERO-Blunt Simple Cloning Kit
- CYCV04 pBLUE-TA Cloning Kit
- CYCV11 pBLUE-T Simple Cloning Kit
- CYCV05 Universal T-Vector Colony PCR Identification Kit
- CYCV06 Blunt-End DNA A-Tailing Kit
- CYCV10 DNA Quick Ligation Kit
- CYCV07 T4 DNA Ligase
- CYCV09 One Tube Mutagenesis Kit
- CYCV2301 ColiPlate Glass Beads (Sterilized)
- CYCC01 TOP10 Competent Cells
- CYCC02 DH5α Competent Cells
- CYCC03 JM109 Competent Cells
- CYCC06 BL21(DE3) Competent Cells
- CYCC08 High-Efficiency Competent Cell Preparation Kit
- CYPC69 RandomMutation Random Mutagenesis Kit (Error-Prone PCR)
- CYCV21 Zero Background pTOPO-TA/Blunt Cloning Kit
- CYCV22 Zero Background pTOPO-TA/Blunt Simple Cloning Kit
- CYPV02 pTOPO-D1 Directional pET Expression Kit