Changyu pTOPO kits clone PCR products for research use: TA (CYCV14) or TA Simple (CYCV15) for Taq A-overhangs; Blunt (CYCV16) or Blunt Simple (CYCV17) for Pfu/KOD/Phusion products. DNA must not be 5′-phosphorylated. Kits ship on ice packs; competent cells are not in the box. Research Use Only.
Choose your pTOPO kit
| Kit | Cat# | Insert ends | For PCR products from |
|---|---|---|---|
| pTOPO-TA | CYCV14 | A-overhangs | Taq-type polymerases (e.g., FastLong) |
| pTOPO-TA Simple | CYCV15 | A-overhangs | Taq-type polymerases, simplified vector backbone |
| pTOPO-Blunt | CYCV16 | Blunt | Proofreading polymerases (e.g., FastHiFi, LongHiFi) — no A-tailing |
| pTOPO-Blunt Simple | CYCV17 | Blunt | Proofreading polymerases, simplified vector backbone |
All four kits: 5-minute room-temperature reaction, 100 bp–5 kb demonstrated inserts (design limit 10 kb), zero-background selection (no blue-white screening), ampicillin resistance, and no competent cells or ligase included (manual). For reference, Thermo’s public TOPO TA pages describe their TA kits as efficient up to about 2–3 kb inserts with a 5-minute workflow — our kits cover a wider stated insert range and skip blue-white screening.
Available product families
pTOPO-TA Cloning Kit
Cat# CYCV14 — TA cloning of Taq products; DNA must not be 5′-phosphorylated. Research Use Only.
pTOPO-TA Simple Cloning Kit
Cat# CYCV15 — TA Simple chemistry; Taq A-overhang fragments; do not 5′-phosphorylate. Research Use Only.
pTOPO-Blunt Cloning Kit
Cat# CYCV16 — Blunt cloning of Pfu/KOD/Phusion products; do not 5′-phosphorylate. Research Use Only.
pTOPO-Blunt Simple Cloning Kit
Cat# CYCV17 — Blunt Simple chemistry; same blunt-end rule as CYCV16. Research Use Only.
Typical research applications
- Cloning Taq PCR products with 3′-A overhangs (CYCV14 / CYCV15)
- Cloning blunt products from Pfu, KOD or Phusion-type polymerases (CYCV16 / CYCV17)
- Inserts tested from about 100 bp to 5 kb; kits are designed for fragments up to 10 kb
- Preparing cloned templates for sequencing
TA, Blunt, or Simple?
- Match the polymerase — TA kits (CYCV14, CYCV15) for Taq A-overhang fragments. Blunt kits (CYCV16, CYCV17) for Pfu/KOD/Phusion products.
- Standard vs Simple — Standard vectors (CYCV14, CYCV16) have common restriction sites flanking the cloning site so you can cut the insert out. Simple vectors (CYCV15, CYCV17) omit those flanking sites to avoid redundant restriction sites in the insert.
- Do not 5′-phosphorylate the DNA — all four kits require free 5′-OH ends. Competent cells are not in the box (vector, 1000 bp control, enhancer only).
- Shipping — ice packs; store at −20°C (12 months in the manuals).
Frequently asked questions
- TA or Blunt? If the amplicon came from Taq (including FastLong CYPC80), use CYCV14 or CYCV15. If it came from a high-fidelity blunt polymerase (FastHiFi CYPC82, LongHiFi CYPC84), use CYCV16 or CYCV17.
- What does Simple mean? The Simple vectors do not carry common restriction sites next to the cloning site. Use them when those sites would collide with sites inside your insert.
- Do the kits include competent cells? No. Listed contents are the pTOPO vector, a 1000 bp control insert, and enhancer. Ask if you also need cells.
- How are kits shipped? Ice packs for every CYCV catalog. Store at −20°C on arrival.
- Are these kits for clinical use? No. Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use.
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All products are for Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use.