CYRN15 RNAfixer is an aqueous RNA stabilizer for fresh animal tissues, cultured cells, and some plant tissues. Immerse the sample immediately — do not freeze it first. In solution, RNA is typically held about 1 day at 37°C, 1 week at 25°C, or 1 month at 4°C. The reagent ships without ice packs. Research Use Only.
Stability at a glance
| Sample | Storage | Stability |
|---|---|---|
| Fresh tissue | 37°C | Up to 1 day |
| Fresh tissue | 25°C (ambient) | Up to 1 week |
| Fresh tissue | 4°C | Up to 1 month |
| Fresh tissue | −20°C / −80°C | Long-term (overnight at 4°C first) |
| RNA viruses (HCV, HIV) | 37°C | Up to 1 month |
| Bacteria (E. coli) | 4°C | Up to 1 month |
| Yeast | 25°C / 4°C | 8 h / 1 week (freeze for longer) |
Sample preservation does not require liquid nitrogen, dry ice, or −80°C freezers. Cut fresh tissue so one side is under 0.5 cm; small organs such as mouse liver, kidney, or spleen can go in whole. Use about 5 volumes of reagent for tissue, 5–10 volumes for cultured cells, or 3 volumes for whole blood. All values are from the CYRN15 manual.
Available product families
RNAfixer Stabilization Solution
Cat# CYRN15 — Room-temperature RNA stabilizer for fresh tissue (do not freeze first); about 1 week at 25°C in solution. Research Use Only.
Typical research applications
- Collecting fresh animal tissue without liquid nitrogen
- Stabilizing cultured cells, and some plant tissues, before RNA extraction
- Holding samples about 1 week at 25°C for ambient shipment
- Longer hold at 4°C (about 1 month) or at −20°C / −80°C after immersion
Handling that actually matters
- Fresh tissue only — immerse immediately after collection. Do not freeze the sample first.
- Times in solution (from the manual) — about 1 day at 37°C, 1 week at 25°C, 1 month at 4°C; longer at −20°C or −80°C.
- The reagent itself — store the liquid at 18–25°C for 12 months. If it separates, warm at 37°C to redissolve. Ships ambient (no ice packs).
- Downstream — RNAfixer-treated samples are used with Changyu Plus RNA Mini kits (CYRN28) and other silica-column RNA protocols. Research Use Only.
Looking for an alternative RNA preservation workflow? Read the CYRN15 purchasing and application questions below.
For a fuller walk-through, read RNAfixer vs RNAlater: RNA Stabilization Reagents Compared.
Frequently asked questions
- How long does RNAfixer preserve samples? In solution: about 1 day at 37°C, 1 week at 25°C, 1 month at 4°C. Do not use a generic “48 hours at room temperature” figure — that is not this product’s manual.
- Can I freeze the tissue first? No. Cut to size and immerse fresh. After immersion, samples may be frozen.
- What sizes exist? CYRN1501 50 ml and CYRN1502 100 ml. Request a quote; there is no list price on this site.
- Is this for diagnostic specimens? No. Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use.
- Why is it sometimes written “RNAfix”? RNAfix and RNAfixer refer to the same reagent — “rnafix” is the common short form used in lab protocols. The full product name is RNAfixer (CYRN15).
- Do I need liquid nitrogen or dry ice? No. Sample preservation does not require liquid nitrogen, dry ice, or −80°C freezers. Immerse fresh tissue at collection; freeze (−20°C / −80°C) only after overnight incubation at 4°C.
- How should I cut tissue, and how much reagent do I need? Cut fresh tissue so one side is under 0.5 cm; small organs such as mouse liver, kidney, or spleen can go in whole. Use about 5 volumes of RNAfixer for tissue, 5–10 volumes for cultured cells, or 3 volumes for whole blood. Blot excess reagent from tissue before extraction.
- Is RNAfixer validated for RNA-seq? The CYRN15 manual does not make an RNA-seq claim. It states the reagent reduces errors in large-scale sample processing and increases comparability between experiments — especially useful for large-scale gene expression profiles. For expression-level studies, run a matched pilot against snap-frozen controls before adopting a preservation workflow.
- How is RNAfixer disposed of? RNAfixer is aqueous and non-toxic. Per the manual, used solution can be disposed of down the drain with tap-water rinsing.
Request a quotation · Email market@changyubio.com · WhatsApp +86 18665577773
All products are for Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use.
Looking for an alternative to your current kit?
Start with the relevant product questions below. Match the sample, method and downstream requirements before switching; protocol settings and kit components are not assumed to be interchangeable.
Alternative-product and purchasing questions
Is CYRN15 RNAfixer an alternative to RNAlater for RNA sample preservation?
CYRN15 RNAfixer is an RNA-stabilization option to evaluate for samples covered by its manual. Check sample type, tissue preparation, storage temperature and holding time before replacing an existing RNAlater preservation workflow.
Can RNAfixer replace an RNA extraction kit or RNAlater-ICE?
RNA stabilization and RNA extraction are different steps. Do not treat CYRN15 as an extraction kit or assume it replaces a product intended specifically for transitioning already-frozen tissue. Follow the CYRN15 instructions for your sample.
How do I request a quotation for CYRN15?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Include the sample type and downstream application.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.