CYCC06 is competent BL21(DE3) E. coli prepared for chemical DNA transformation. Transformation efficiency is stated as up to 10^7 with pUC19 plasmid, and efficiency does not change after several months at -70 °C. Each tube can be aliquoted as needed to cut cost.
Catalog no. CYCC06 · For research use only
Key features
- Chemically competent BL21(DE3) cells for DNA transformation
- Transformation efficiency up to 10^7 with pUC19
- Efficiency unchanged for several months at -70 °C
- Tubes can be aliquoted for use to reduce cost
- Host for T7 RNA polymerase expression systems; the T7 polymerase gene is under the lacUV5 promoter and is suited to non-toxic protein expression
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCC0601 | BL21(DE3) Competent Cells | 5 x 100 μL |
| CYCC0602 | BL21(DE3) Competent Cells | 10 x 100 μL |
| CYCC0603 | BL21(DE3) Competent Cells | 20 x 100 μL |
Specifications
| Strain | BL21(DE3) |
|---|---|
| Format | Chemically competent cells, 100 μL tubes |
| Efficiency | Up to 10^7 (pUC19) |
| Storage | -70 °C |
| Genotype highlights | ompT, hsdSB, gal, dcm, (DE3) |
| Use | Research use only |
Applications
- Plasmid transformation
- Recombinant protein expression with T7-driven vectors
- Routine molecular biology laboratory use
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
BL21(DE3) Competent Cells FAQ
What transformation efficiency can I expect?
Up to 10^7 with pUC19 plasmid.
How should the cells be stored?
At -70 °C. Efficiency stays unchanged for several months at -70 °C.
Can I aliquot the tubes?
Yes, each tube can be divided as needed to lower experiment cost.
How do I order CYCC06?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.