CYCV22 is a TOPO cloning kit for any PCR product without 5′ phosphates, compatible with A-ends and blunt ends. It uses topoisomerase rather than T4 ligase to join DNA in seconds to minutes with close to 100% efficiency, and a suicide-gene system gives zero background, so blue-white and colony PCR screening are not needed. Vector is under 2 kb and inserts of up to 10 kb can be ligated. The vector has no multiple cloning sites flanking the insert site (Simple), so restriction sites can be added on the PCR primers.
Catalog no. CYCV22 · For research use only
Request a quote · Download manual (PDF)
Key features
- Topoisomerase ligation in seconds to minutes with close to 100% efficiency
- Vector smaller than 2 kb, giving more transformants and better ligation of large inserts; fragments up to 10 kb can be ligated
- Ampicillin resistance needs only 0-10 minutes recovery after transformation; recovery can be skipped for routine inserts, so ligation to plating takes about 15 minutes; for long inserts or low-efficiency competent cells, recover 30–60 minutes as the manual describes
- Suicide-gene zero-background selection; no blue-white or colony PCR screening, and a randomly picked colony nearly always has an insert
- No multiple cloning sites at the insert site (Simple version), so added primer sites cut cleanly
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV2201 | Zero Background pTOPO-TA/Blunt Simple Cloning Kit | 40 reactions (5 μL) |
| CYCV2202 | Zero Background pTOPO-TA/Blunt Simple Cloning Kit | 160 reactions (5 μL) |
Specifications
| Format | TOPO vector cloning kit (Simple, no MCS) |
|---|---|
| Insert | any PCR product without 5′ phosphates, compatible with A-ends and blunt ends |
| Ligation | Seconds to minutes |
| Max insert | 10 kb |
| Selection | Ampicillin; suicide-gene zero background |
| Sequencing primers | M13F/M13R universal primers (not M13(-47)/M13(-48)) |
| Storage | -20 °C; stable for at least 12 months |
| Shipping | Ice packs |
| Use | Research use only |
Applications
- Fast cloning of PCR products without screening
- Cloning long fragments up to 10 kb
- Subcloning with restriction sites added on PCR primers
Ordering
International ordering — CYCV22
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Shipping conditions are confirmed per item on the quotation.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV22 — tell us the quantity and destination city.
Zero Background pTOPO-TA/Blunt Simple Cloning Kit FAQ
What is the reaction format?
Each unit is a 5 μL reaction; 40 or 160 reactions per kit. At 10 μL per ligation the kit covers half as many ligations.
How do I sequence the insert?
Use M13F/M13R universal primers, not M13(-47)/M13(-48). Colony PCR can use the same primers.
Is blue-white screening needed?
No. The suicide-gene system gives zero background, so a randomly picked colony nearly always carries an insert.
How do I order CYCV22?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.