CYCV05 is a colony PCR kit for identifying positive T-vector clones that carry an insert. Its universal T-vector primers work with common T vectors including the pGEM, pUC and pBluescript series, so one kit replaces several vector-specific ones. The primers sit at least 150 bp apart before any insert, so a small insert band can be told from primer dimer. A one-tube PCR MasterMix system gives results in about an hour.
Catalog no. CYCV05 · For research use only
Key features
- Universal primers for common T vectors, including pGEM, pUC and pBluescript series
- Primers at least 150 bp apart without an insert, so insert bands are distinguishable from primer dimers, even for inserts around 100 bp or smaller
- One-tube PCR MasterMix: just add a single colony
- Result after about 1 hour of PCR plus gel electrophoresis
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV0501 | Universal T-Vector Colony PCR Identification Kit | 80-100 reactions |
| CYCV0502 | Universal T-Vector Colony PCR Identification Kit | 400-500 reactions |
Specifications
| Format | Colony PCR kit with premix |
|---|---|
| Vectors | pGEM, pUC, pBluescript T vectors |
| Primer spacing | >=150 bp without insert |
| Time | About 1 hour plus electrophoresis |
| Extension (inserts <1 kb) | 30-45 seconds |
| Storage | -20 °C |
| Use | Research use only |
Applications
- Screening T-vector clones for inserts
- Colony PCR of small and large inserts
- Avoiding false positives from primer dimer
Ordering
International ordering — CYCV05
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Shipping conditions are confirmed per item on the quotation.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV05 — tell us the quantity and destination city.
Universal T-Vector Colony PCR Identification Kit FAQ
Which vectors does it suit?
Common T vectors, including the pGEM, pUC and pBluescript series.
Why are the primers placed this way?
With T3, T7 or SP6 primers the product with no insert is very short, so primer dimer can look like an insert of around 100 bp. These primers are at least 150 bp apart, which separates the two.
Any tips?
Mark single colonies before picking, pick a single colony without too much cell material, and set extension time by insert size: 30-45 seconds for inserts under 1 kb, scaled up for longer ones.
How do I order CYCV05?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.
Product manual
CYCV05 instructions for use (English, PDF).