CYCV12 is a seamless cloning kit that uses homologous recombination rather than T4 ligase and does not depend on restriction sites. A special recombinase joins a linearized vector and blunt-end PCR fragments carrying 16–25 bp overlaps at each end, allowing efficient seamless cloning of 1 to 5 fragments.
Catalog no. CYCV12 · For research use only
Request a quote · Download manual (PDF)
Key features
- Homologous recombination through 16–25 bp overlaps; no T4 ligase
- Assembles 1 to 5 PCR fragments (long or short) into a vector in 30 minutes at 50 °C (60 minutes for more than 3 fragments)
- Independent of restriction site availability and of blunt versus sticky ends
- Seamless: no unwanted bases added at the insertion point
- Positive rate above 95%; stored at -20 °C; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV1201 | Seamless Assembly and Cloning Kit | 10 reactions |
Specifications
| Method | Homologous recombination, 1-5 fragments |
|---|---|
| Overlap | 16–25 bp each end |
| Fragments | Blunt-end PCR products |
| Time | 50 °C, 30 minutes; 60 minutes for more than 3 fragments |
| Positive rate | >95% |
| Storage | -20 °C, avoid repeated freeze-thaw |
| Use | Research use only |
Applications
- Assembling several PCR fragments into one vector
- Seamless single-fragment cloning
- Restriction-site-free construct building
Ordering
International ordering — CYCV12
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Storage: -20 °C, avoid repeated freeze-thaw. Shipping temperature is confirmed per item.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV12 — tell us the quantity and destination city.
Seamless Assembly and Cloning Kit FAQ
How many fragments can I assemble?
One to five PCR fragments.
Do I need restriction sites?
No. Cloning is not limited by restriction site availability or by blunt/sticky ends.
How much overlap do I design?
16–25 bp of overlap at the ends of each PCR fragment and the linearized vector.
How do I order CYCV12?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
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