2× master mix built on CYA8 FastHiFi DNA polymerase — a genetically engineered Pfu derivative with greatly improved amplification speed, fidelity and yield. The preferred choice for ultra-fidelity, fast amplification of non-long fragments.
Key Features
- Engineered Pfu derivative (CYA8) with superior fidelity and speed
- Greatly improved amplification rate, fidelity and yield
- 2× ready-to-use format: add template, primers and water
- Ideal for downstream cloning and sequencing
Specifications
| Format | 2× mix: CYA8 FastHiFi DNA Polymerase, dNTPs, optimized reaction buffer |
| Polymerase | Genetically engineered Pfu (FastHiFi) — high fidelity |
| Storage | -20°C; avoid repeated freeze-thaw |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- High-fidelity PCR from complex genomic DNA (manual: not more than 3 kb on complex gDNA).
- Faster Pfu-type PCR (manual: 2–4 kb/min). Blunt-end products — use a blunt cloning kit, not TA.
- Simple templates (plasmid, phage) when a blunt, high-fidelity insert is required.
Performance at a glance
| Max amplicon | Not more than 3 kb on complex genomic DNA; not more than 6 kb on simple genomes, plasmids or phage DNA (manual) |
| Speed | 2–4 kb/min — up to 4–8× of Pfu; 15–20 s/kb on simple templates, 30–45 s/kb on complex genomes (manual) |
| Fidelity | Up to 54× of traditional Taq DNA polymerase |
| Yield | Up to 1.5–2× of traditional Pfu |
| Human genomic DNA evidence | Hα1AT 2.6 kb amplified from 50 ng human gDNA in 50 µl, extension 30 s/kb (manual, Figure 1) |
| Template input (50 µl) | Plasmid 0.1–10 ng; bacterial genome 10–100 ng; human genome 50–150 ng; cDNA 1–5 µl |
| High-GC / complex templates | Denaturation up to 98°C (CYA8 activity unaffected); DMSO 1–8% or betaine 1.0–1.7 M; touchdown PCR |
| Product ends | Blunt — pair with pTOPO Blunt vectors, not TA cloning |
| Storage / pack sizes | −20°C, 1 year; CYPC8201 (1 ml) · CYPC8202 (5 ml) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 1 year). Avoid repeated freeze–thaw.
- Typical length: not more than ~3 kb on complex genomic DNA; not more than ~6 kb on simple genomes, plasmids, or phage DNA.
- Products are blunt-ended. Extension: about 15–20 s/kb on simple templates; 30–45 s/kb on complex genomes.
Who sets these specifications
Changyu Bio supplies Cat# CYPC82 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
FastHiFi FAQ
How fast is FastHiFi compared with a standard Pfu mix?
FastHiFi amplifies at 2–4 kb/min, up to 4–8× the speed of Pfu, with fidelity up to 54× of traditional Taq and yield up to 1.5–2× of traditional Pfu (manual).
What template lengths can FastHiFi handle?
Per the manual: not more than ~3 kb on complex genomic DNA, and not more than ~6 kb on simple genomes, plasmids or phage DNA. Recommended 50-µl inputs: plasmid 0.1–10 ng, bacterial genome 10–100 ng, human genome 50–150 ng, or 1–5 µl cDNA from an RT reaction.
Are FastHiFi products blunt-ended?
Yes. Products are blunt-ended — use a blunt-end cloning workflow such as our pTOPO Blunt kits, not TA cloning. After purification, products also work for digestion, ligation or sequencing.
How do I amplify high-GC or complex templates?
Raise pre-denaturation and denaturation to 98°C (CYA8 is heat-resistant and its activity is not influenced at 98°C). If amplification is still poor, add DMSO to 1–8% final (step up in 1% increments) or betaine to 1.0–1.7 M final, together with touchdown PCR.
What cycling conditions does the manual propose?
95°C 3 min → 95°C 10 s / 55°C 10–15 s / 72°C 15–30 s per kb × 30 cycles → final extension 72°C 2–5 min. Choose 30–45 s/kb on complex genomes such as human.
Cat# CYPC82 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.
Alternative-product and purchasing questions
Is CYPC82 an alternative to evaluate for a Q5 or Phusion high-fidelity PCR workflow?
CYPC82 FastHiFi is a high-fidelity PCR option to evaluate for targets within its documented range. Match template, amplicon length and downstream requirements. This application-level choice does not mean it uses the same enzyme or has demonstrated the same error rate as Q5 or Phusion.
What changes should I check when moving to CYPC82?
Use the CYPC82 buffer and cycling instructions, and assess amplification and sequence accuracy as appropriate to your experiment. Its documented products are blunt-ended, so choose a compatible cloning route rather than assuming direct TA cloning.
How do I request a quotation for CYPC82?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Include the instrument or cycling workflow where relevant.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.