2× master mix built on CYA8 FastHiFi DNA polymerase — a genetically engineered Pfu derivative with greatly improved amplification speed, fidelity and yield. The preferred choice for ultra-fidelity, fast amplification of non-long fragments.
Key Features
- Engineered Pfu derivative (CYA8) with superior fidelity and speed
- Greatly improved amplification rate, fidelity and yield
- 2× ready-to-use format: add template, primers and water
- Ideal for downstream cloning and sequencing
Specifications
| Format | 2× mix: CYA8 FastHiFi DNA Polymerase, dNTPs, optimized reaction buffer |
| Polymerase | Genetically engineered Pfu (FastHiFi) — high fidelity |
| Storage | -20°C; avoid repeated freeze-thaw |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- High-fidelity PCR from complex genomic DNA (manual: not more than 3 kb on complex gDNA).
- Faster Pfu-type PCR (manual: 2–4 kb/min). Blunt-end products — use a blunt cloning kit, not TA.
- Simple templates (plasmid, phage) when a blunt, high-fidelity insert is required.
Performance at a glance
| Max amplicon | Not more than 3 kb on complex genomic DNA; not more than 6 kb on simple genomes, plasmids or phage DNA (manual) |
| Speed | 2–4 kb/min — up to 4–8× of Pfu; 15–20 s/kb on simple templates, 30–45 s/kb on complex genomes (manual) |
| Fidelity | Up to 54× of traditional Taq DNA polymerase |
| Yield | Up to 1.5–2× of traditional Pfu |
| Human genomic DNA evidence | Hα1AT 2.6 kb amplified from 50 ng human gDNA in 50 µl, extension 30 s/kb (manual, Figure 1) |
| Template input (50 µl) | Plasmid 0.1–10 ng; bacterial genome 10–100 ng; human genome 50–150 ng; cDNA 1–5 µl |
| High-GC / complex templates | Denaturation up to 98°C (CYA8 activity unaffected); DMSO 1–8% or betaine 1.0–1.7 M; touchdown PCR |
| Product ends | Blunt — pair with pTOPO Blunt vectors, not TA cloning |
| Storage / pack sizes | −20°C, 1 year; CYPC8201 (1 ml) · CYPC8202 (5 ml) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 1 year). Avoid repeated freeze–thaw.
- Typical length: not more than ~3 kb on complex genomic DNA; not more than ~6 kb on simple genomes, plasmids, or phage DNA.
- Products are blunt-ended. Extension: about 15–20 s/kb on simple templates; 30–45 s/kb on complex genomes.
Who sets these specifications
Changyu Bio manufactures Cat# CYPC82 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
FastHiFi FAQ
How fast is FastHiFi compared with a standard Pfu mix?
FastHiFi amplifies at 2–4 kb/min, up to 4–8× the speed of Pfu, with fidelity up to 54× of traditional Taq and yield up to 1.5–2× of traditional Pfu (manual).
What template lengths can FastHiFi handle?
Per the manual: not more than ~3 kb on complex genomic DNA, and not more than ~6 kb on simple genomes, plasmids or phage DNA. Recommended 50-µl inputs: plasmid 0.1–10 ng, bacterial genome 10–100 ng, human genome 50–150 ng, or 1–5 µl cDNA from an RT reaction.
Are FastHiFi products blunt-ended?
Yes. Products are blunt-ended — use a blunt-end cloning workflow such as our pTOPO Blunt kits, not TA cloning. After purification, products also work for digestion, ligation or sequencing.
How do I amplify high-GC or complex templates?
Raise pre-denaturation and denaturation to 98°C (CYA8 is heat-resistant and its activity is not influenced at 98°C). If amplification is still poor, add DMSO to 1–8% final (step up in 1% increments) or betaine to 1.0–1.7 M final, together with touchdown PCR.
What cycling conditions does the manual propose?
95°C 3 min → 95°C 10 s / 55°C 10–15 s / 72°C 15–30 s per kb × 30 cycles → final extension 72°C 2–5 min. Choose 30–45 s/kb on complex genomes such as human.
Cat# CYPC82 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.