2× master mix containing CYF8 FastLong DNA Polymerase, dNTPs and optimized buffer. Amplifies long fragments quickly, includes a red tracer dye for quicker electrophoresis, and products carry polyA ends ready for pTOPO TA cloning.
Key Features
- FastLong enzyme for rapid amplification of long targets
- Red tracer dye — for quicker electrophoresis
- Products with polyA ends clone directly into pTOPO TA vectors
- 2× ready-to-use format: add template, primers and water
Specifications
| Format | 2× mix: CYF8 FastLong DNA Polymerase, dNTPs, optimized reaction buffer |
| Convenience | Contains red tracer dye for quicker electrophoresis |
| Cloning | PolyA-tailed products can be used directly with pTOPO vectors |
| Storage | -20°C; avoid repeated freeze-thaw |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- Fast PCR around 10 kb (manual: about 5–10 s/kb) from DNA or cDNA templates.
- Templates with high GC or complicated structure.
- Red tracer dye for quicker electrophoresis. Products have polyA ends for TA cloning.
Performance at a glance
| Max amplicon | About 10 kb (per manual) |
| Speed | 5–10 s/kb; 5 s/kb for simple or plasmid templates, 15–20 s/kb for complex templates |
| Fidelity | Up to 3–6× that of traditional Taq DNA polymerase |
| Human genomic DNA evidence | 2.6 kb (Hα1AT) and 6 kb (β-globin) amplified from 50 ng human genomic DNA in 50 μl reactions (manual, Figure 1) |
| Template | DNA and cDNA |
| High-GC / complex templates | Denaturation up to 98°C (short fragments); DMSO 1–8% or betaine 1.0–1.7 M; touchdown PCR |
| Product ends | polyA — direct cloning into pTOPO vectors, no A-tailing needed |
| Storage | −20°C, 2 years |
| Pack sizes | CYPC8001 1 ml · CYPC8002 5 ml |
All values from the CYPC80 manual (PDF).
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 2 years). Avoid repeated freeze–thaw.
- Very fast cycling: about 5 s/kb is for simple or plasmid templates; use longer extension and more cycles on complex templates.
- Products are polyA-tailed (suitable for TA cloning). For high-GC templates, denaturation may be raised to 98°C as in the manual.
Who sets these specifications
Changyu Bio supplies Cat# CYPC80 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
FastLong FAQ
What is the longest amplicon FastLong supports?
About 10 kb per the manual. The manual shows gel evidence for 2.6 kb (Hα1AT) and 6 kb (β-globin) fragments from 50 ng human genomic DNA. For longer targets, validate on your template or split the amplicon into overlapping fragments.
Can I clone FastLong PCR products directly?
Yes. Products carry polyA ends and clone directly into pTOPO TA vectors without A-tailing. After purification, products can also be used for digestion, ligation, or fluorescence sequencing.
How do I amplify high-GC or complex templates?
For short fragments, denaturation may be raised to 98°C. For long fragments, keep the temperature and instead extend pre-denaturation (e.g., 5 min) and denaturation time. Add DMSO 1–8% or betaine 1.0–1.7 M, and use touchdown PCR.
Cat# CYPC80 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery worldwide.
Alternative-product and purchasing questions
Can CYPC80 be considered instead of a routine Taq-based PCR master mix?
CYPC80 FastLong is a PCR master-mix option to review for routine amplification and A-tailed cloning workflows. Match the target length, template and required fidelity to the manual rather than assuming every Taq or high-fidelity mix is interchangeable.
Does CYPC80 replace Q5 or Phusion for every cloning application?
No universal substitution is established. CYPC80 produces A-tailed products, while end requirements and fidelity are important in proofreading-PCR workflows. Review CYPC82 or CYPC84 when those requirements point to a high-fidelity, blunt-end route.
How do I request a quotation for CYPC80?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Include the instrument or cycling workflow where relevant.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.