2× master mix containing CYF8 FastLong DNA Polymerase, dNTPs and optimized buffer. Amplifies long fragments quickly, includes a red tracer dye for quicker electrophoresis, and products carry polyA ends ready for pTOPO TA cloning.
Key Features
- FastLong enzyme for rapid amplification of long targets
- Red tracer dye — for quicker electrophoresis
- Products with polyA ends clone directly into pTOPO TA vectors
- 2× ready-to-use format: add template, primers and water
Specifications
| Format | 2× mix: CYF8 FastLong DNA Polymerase, dNTPs, optimized reaction buffer |
| Convenience | Contains red tracer dye for quicker electrophoresis |
| Cloning | PolyA-tailed products can be used directly with pTOPO vectors |
| Storage | -20°C; avoid repeated freeze-thaw |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- Fast PCR around 10 kb (manual: about 5–10 s/kb) from DNA or cDNA templates.
- Templates with high GC or complicated structure.
- Red tracer dye for quicker electrophoresis. Products have polyA ends for TA cloning.
Performance at a glance
| Max amplicon | About 10 kb (per manual) |
| Speed | 5–10 s/kb; 5 s/kb for simple or plasmid templates, 15–20 s/kb for complex templates |
| Fidelity | Up to 3–6× that of traditional Taq DNA polymerase |
| Human genomic DNA evidence | 2.6 kb (Hα1AT) and 6 kb (β-globin) amplified from 50 ng human genomic DNA in 50 μl reactions (manual, Figure 1) |
| Template | DNA and cDNA |
| High-GC / complex templates | Denaturation up to 98°C (short fragments); DMSO 1–8% or betaine 1.0–1.7 M; touchdown PCR |
| Product ends | polyA — direct cloning into pTOPO vectors, no A-tailing needed |
| Storage | −20°C, 2 years |
| Pack sizes | CYPC8001 1 ml · CYPC8002 5 ml |
All values from the CYPC80 manual (PDF).
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 2 years). Avoid repeated freeze–thaw.
- Very fast cycling: about 5 s/kb is for simple or plasmid templates; use longer extension and more cycles on complex templates.
- Products are polyA-tailed (suitable for TA cloning). For high-GC templates, denaturation may be raised to 98°C as in the manual.
Who sets these specifications
Changyu Bio manufactures Cat# CYPC80 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
FastLong FAQ
What is the longest amplicon FastLong supports?
About 10 kb per the manual. The manual shows gel evidence for 2.6 kb (Hα1AT) and 6 kb (β-globin) fragments from 50 ng human genomic DNA. For longer targets, validate on your template or split the amplicon into overlapping fragments.
Can I clone FastLong PCR products directly?
Yes. Products carry polyA ends and clone directly into pTOPO TA vectors without A-tailing. After purification, products can also be used for digestion, ligation, or fluorescence sequencing.
How do I amplify high-GC or complex templates?
For short fragments, denaturation may be raised to 98°C. For long fragments, keep the temperature and instead extend pre-denaturation (e.g., 5 min) and denaturation time. Add DMSO 1–8% or betaine 1.0–1.7 M, and use touchdown PCR.
Cat# CYPC80 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery worldwide.