CYCC03 is competent JM109 E. coli prepared for chemical DNA transformation. Transformation efficiency is stated as up to 10^8 with pUC19 plasmid, and efficiency does not change after several months at -70 °C. Each tube can be aliquoted as needed to cut cost.
Catalog no. CYCC03 · For research use only
Key features
- Chemically competent JM109 cells for DNA transformation
- Transformation efficiency up to 10^8 with pUC19
- Efficiency unchanged for several months at -70 °C
- Tubes can be aliquoted for use to reduce cost
- recA1 and endA1 mutations prevent modification of cloned DNA and recombination with host chromosomal DNA, improving plasmid yield and quality
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCC0301 | JM109 Competent Cells | 5 x 100 μL |
| CYCC0302 | JM109 Competent Cells | 10 x 100 μL |
| CYCC0303 | JM109 Competent Cells | 20 x 100 μL |
Specifications
| Strain | JM109 |
|---|---|
| Format | Chemically competent cells, 100 μL tubes |
| Efficiency | Up to 10^8 (pUC19) |
| Storage | -70 °C |
| Genotype highlights | endA1, recA1, hsdR17, supE44 |
| Use | Research use only |
Applications
- Plasmid transformation
- Cloning of ligation products
- Routine molecular biology laboratory use
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
JM109 Competent Cells FAQ
What transformation efficiency can I expect?
Up to 10^8 with pUC19 plasmid.
How should the cells be stored?
At -70 °C. Efficiency stays unchanged for several months at -70 °C.
Can I aliquot the tubes?
Yes, each tube can be divided as needed to lower experiment cost.
How do I order CYCC03?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.