CYCV07 is T4 DNA Ligase, purified from E. coli expressing the T4 DNA ligase gene and supplied at 400 U/μL. It forms phosphodiester bonds between adjacent 5′-phosphate and 3′-hydroxyl ends. It ligates blunt or sticky ends and repairs single-strand nicks in double-stranded DNA, RNA and DNA/RNA hybrids.
Catalog no. CYCV07 · For research use only
Request a quote · Download manual (PDF)
Key features
- Catalyzes joining of 5′-phosphate and 3′-hydroxyl DNA ends
- Ligates blunt-end or sticky-end DNA
- Repairs nicks in dsDNA, RNA and DNA/RNA hybrids
- Supplied at 400 U/μL; -20 °C storage, 12 months
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV0701 | T4 DNA Ligase | 40 KU |
| CYCV0702 | T4 DNA Ligase | 200 KU |
Specifications
| Enzyme | T4 DNA Ligase |
|---|---|
| Concentration | 400 U/μL |
| Activity | Blunt and sticky end ligation; nick repair |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- Ligation of DNA fragments into vectors
- Blunt and sticky end ligation
- Repair of nicks in dsDNA, RNA and DNA/RNA hybrids
Ordering
International ordering — CYCV07
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Storage: -20 °C, 12 months. Shipping temperature is confirmed per item.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV07 — tell us the quantity and destination city.
T4 DNA Ligase FAQ
What does T4 DNA Ligase do?
It catalyzes formation of phosphodiester bonds between adjacent 5′-phosphate and 3′-hydroxyl ends of DNA.
Which ends can it ligate?
Blunt or sticky ends, and it also repairs single-strand nicks in double-stranded DNA, RNA and DNA/RNA hybrids.
How is it stored?
At -20 °C for 12 months; supplied at 400 U/μL.
How do I order CYCV07?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
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