CYCV08 is a zero-background blunt-end cloning kit that uses a suicide-gene positive selection system to clone blunt PCR products and other blunt DNA, phosphorylated or not. Ligation takes 5 minutes and gives over 95% positive recombinant clones. The vector pZERO-Blunt carries a lethal gene that is disrupted by a successful insert, so empty self-ligated vectors do not survive.
Catalog no. CYCV08 · For research use only
Request a quote · Download manual (PDF)
Key features
- Suicide-gene positive selection; no blue-white screening needed
- 5 minute ligation with over 95% positive recombinants
- Works with phosphorylated or unphosphorylated blunt DNA; PCR products from proofreading polymerases such as Pfu go straight into the vector
- BglII sites flank the multiple cloning site for mapping and insert manipulation; T7 promoter for in vivo or in vitro transcription and insert sequencing
- Ligation product transforms common E. coli strains directly; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV0801 | pZERO-Blunt Cloning Kit | 20 reactions |
| CYCV0802 | pZERO-Blunt Cloning Kit | 40 reactions |
Specifications
| Vector | pZERO-Blunt (suicide gene, MCS) |
|---|---|
| Insert | Blunt-end DNA, phosphorylated or not |
| Ligation time | 5 minutes |
| Positive rate | >95% |
| Features | BglII sites flanking the MCS; T7 promoter |
| Storage | -20 °C; stable for 6 months |
| Use | Research use only |
Applications
- Cloning blunt-end PCR products amplified by proofreading polymerase
- Cloning any blunt DNA fragment
- In vitro or in vivo transcription from the T7 promoter
Ordering
International ordering — CYCV08
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Shipping conditions are confirmed per item on the quotation.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV08 — tell us the quantity and destination city.
pZERO-Blunt Cloning Kit FAQ
How does the zero-background selection work?
The vector contains a lethal suicide gene. A successful insert disrupts it so recombinant cells grow, while self-ligated empty vector expresses the gene and the cells die.
Which inserts work?
Blunt-end PCR products from proofreading polymerases such as Pfu, and any blunt DNA fragment, phosphorylated or not.
Which host strains can I use?
Common E. coli strains; the ligation product can be transformed directly.
How do I order CYCV08?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
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