Changyu Bio pTOPO-Blunt Simple Kit carton, Cat# CYCV17

pTOPO-Blunt Simple Cloning Kit | 5-Minute Zero-Background Blunt Cloning (up to 10 kb)

Simplified zero-background blunt-end cloning kit for high-fidelity PCR products (KOD, Pfu, Phusion) and end-polished DNA fragments up to 10 kb. Minimal components, maximal convenience.

Key Features

  • Simplified minimal-component format
  • 5-minute topoisomerase ligation at room temperature
  • Zero-background blunt-end vector
  • Fragments up to 10 kb

Specifications

Pack sizes20 rxns (CYCV1701) / 80 rxns (CYCV1702)
ComponentspTOPO-Blunt Simple Vector (30 ng/µl), 1000 bp Control, 10× Enhancer
Storage-20°C; stable for 12 months
ShippingIce packs; store at the listed temperature on arrival

Typical Applications

  • Blunt cloning up to 10 kb using the Simple vector (no common flanking restriction sites).
  • High-fidelity blunt PCR inserts; 5-minute ligation; 1000 bp control insert.
  • DNA must not be 5′-phosphorylated. Competent cells are not included.

Performance at a glance

Insert sizeUp to 10 kb (design limit); 100 bp – 5 kb demonstrated (10–150 ng)
Insert amount guide100–1000 bp → 10–40 ng; 1000–2000 bp → 40–80 ng (see manual for 2–5 kb row)
Reaction5 minutes at room temperature (15–30°C), 10 µl system
SelectionZero background — no blue-white screening needed
Vector1865 bp, high-copy, pUC ori, AmpR
Flanking restriction sitesNo common restriction sites (simple vector)
Preferred insert endsBlunt ends from high-fidelity polymerases (KOD, Phusion, Pfu); must NOT be 5′-phosphorylated
Storage−20°C, stable 12 months
Pack sizes20 rxns (CYCV1701) / 80 rxns (CYCV1702)

All values from the CYCV17 manual (PDF).

Limits and handling

  • For laboratory research use only. Not for diagnostic or therapeutic use.
  • Shipped with ice packs. Store at the temperature in the specifications table on arrival.
  • Store at −20°C (manual: 12 months).
  • DNA fragments must not be 5′-phosphorylated. Blunt ends from high-fidelity enzymes (KOD, Pfu, Phusion) are preferred. Inserts up to 10 kb.
  • Incubate the cloning reaction no more than 5 minutes at 15–30°C. Mix competent cells gently — do not pipette up and down.

Who sets these specifications

Changyu Bio manufactures Cat# CYCV17 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.

Cat# CYCV17 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.

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