Simplified zero-background blunt-end cloning kit for high-fidelity PCR products (KOD, Pfu, Phusion) and end-polished DNA fragments up to 10 kb. Minimal components, maximal convenience.
Key Features
- Simplified minimal-component format
- 5-minute topoisomerase ligation at 25–37 °C (10–15 minutes at 37 °C for long inserts)
- Zero-background blunt-end vector
- Fragments up to 10 kb
Specifications
| Pack sizes | 20 × 10 µl or 40 × 5 µl (CYCV1701) / 80 × 10 µl or 160 × 5 µl (CYCV1702) |
| Components | pTOPO-Blunt Simple Vector (30 ng/µl), 1000 bp Control, 10× Enhancer |
| Storage | -20°C; stable for 12 months |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- Blunt cloning up to 10 kb using the Simple vector (no common flanking restriction sites).
- High-fidelity blunt PCR inserts; 5-minute ligation; 1000 bp control insert.
- DNA must not be 5′-phosphorylated. Competent cells are not included.
Performance at a glance
| Insert size | Up to 10 kb (design limit); amounts given for 100 bp – 5 kb (10–180 ng) |
| Insert amount guide | 100–1000 bp → 10–40 ng; 1000–2000 bp → 40–80 ng; 2000–5000 bp → 80–180 ng |
| Reaction | 5 minutes at 25–37°C (37°C recommended), 10 µl system; 10–15 minutes at 37°C for long or difficult inserts |
| Selection | Zero background — no blue-white screening needed |
| Vector | 1865 bp, high-copy, pUC ori, AmpR |
| Flanking restriction sites | No common restriction sites (simple vector) |
| Preferred insert ends | Blunt ends from high-fidelity polymerases (KOD, Phusion, Pfu); must NOT be 5′-phosphorylated |
| Storage | −20°C, stable 12 months |
| Pack sizes | 20 × 10 µl or 40 × 5 µl (CYCV1701) / 80 × 10 µl or 160 × 5 µl (CYCV1702) |
All values from the CYCV17 manual (PDF).
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 12 months).
- DNA fragments must not be 5′-phosphorylated. Blunt ends from high-fidelity enzymes (KOD, Pfu, Phusion) are preferred. Inserts up to 10 kb.
- Set up the reaction at room temperature, not on ice; incubate 5 minutes at 25–37°C (10–15 minutes at 37°C for long inserts). Mix competent cells gently — do not pipette up and down.
Who sets these specifications
Changyu Bio supplies Cat# CYCV17 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
Cat# CYCV17 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.
International ordering — CYCV17
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Shipped with ice packs; store at −20 °C on arrival.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV17 — tell us the quantity and destination city.
Alternative-product and purchasing questions
Can CYCV17 replace the blunt-end PCR-capture step in a TOPO-style workflow?
CYCV17 pTOPO-Blunt Simple is an option to evaluate for blunt-ended insert capture. Match its Simple vector design to your downstream analysis; do not assume it is the same vector as an Invitrogen Zero Blunt product.
What should I confirm before choosing CYCV17?
Confirm the PCR product has the required blunt ends, review the vector map and selection method, and check the restrictions of the cloning protocol. Select the regular pTOPO-Blunt option if its vector features better suit your next step.
How do I request a quotation for CYCV17?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Tell us the insert-end type and vector features you require.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.