Simplified zero-background blunt-end cloning kit for high-fidelity PCR products (KOD, Pfu, Phusion) and end-polished DNA fragments up to 10 kb. Minimal components, maximal convenience.
Key Features
- Simplified minimal-component format
- 5-minute topoisomerase ligation at room temperature
- Zero-background blunt-end vector
- Fragments up to 10 kb
Specifications
| Pack sizes | 20 rxns (CYCV1701) / 80 rxns (CYCV1702) |
| Components | pTOPO-Blunt Simple Vector (30 ng/µl), 1000 bp Control, 10× Enhancer |
| Storage | -20°C; stable for 12 months |
Typical Applications
- Cloning of blunt-end PCR products
- High-throughput blunt cloning
- Routine subcloning
Cat# CYCV17 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.