Simplified zero-background TA cloning kit: fewer components, same fast topoisomerase-mediated ligation of Taq PCR products up to 10 kb. Ideal for high-throughput and routine cloning when minimal handling matters.
Key Features
- Minimal-component simplified format
- 5-minute topoisomerase ligation at room temperature
- Zero-background TA vector system
- Fragments up to 10 kb with 3′-A overhangs
Specifications
| Pack sizes | 20 rxns (CYCV1501) / 80 rxns (CYCV1502) |
| Components | pTOPO-T Simple Vector (30 ng/µl), 1000 bp Control, 10× Enhancer |
| Storage | -20°C; stable for 12 months |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- TA cloning of Taq A-overhang inserts up to 10 kb using the Simple vector (no common flanking restriction sites).
- 5 minutes at 15–30°C; 1000 bp control insert included.
- DNA must not be 5′-phosphorylated. Competent cells are not included.
Performance at a glance
| Insert size | Up to 10 kb (design limit); 100 bp – 5 kb demonstrated (10–150 ng) |
| Insert amount guide | 100–1000 bp → 10–40 ng; 1000–2000 bp → 40–80 ng; 2000–5000 bp → 80–150 ng |
| Reaction | 5 minutes at room temperature (15–30°C), 10 µl system |
| Selection | Zero background — no blue-white screening needed |
| Vector | 1865 bp, high-copy, pUC ori, AmpR |
| Flanking restriction sites | No common restriction sites (simple vector) |
| Preferred insert ends | 3′-A overhang from Taq PCR products; must NOT be 5′-phosphorylated |
| Storage | −20°C, stable 12 months |
| Pack sizes | 20 rxns (CYCV1501) / 80 rxns (CYCV1502) |
All values from the CYCV15 manual (PDF).
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 12 months).
- DNA fragments must not be 5′-phosphorylated. TA (A-overhang) ends from Taq are preferred. Inserts up to 10 kb.
- Incubate the cloning reaction no more than 5 minutes at 15–30°C. Mix competent cells gently — do not pipette up and down.
Who sets these specifications
Changyu Bio manufactures Cat# CYCV15 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
Cat# CYCV15 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.