Simplified zero-background TA cloning kit: fewer components, same fast topoisomerase-mediated ligation of Taq PCR products up to 10 kb. Ideal for high-throughput and routine cloning when minimal handling matters.
Key Features
- Minimal-component simplified format
- 5-minute topoisomerase ligation at 25–37 °C (10–15 minutes at 37 °C for long inserts)
- Zero-background TA vector system
- Fragments up to 10 kb with 3′-A overhangs
Specifications
| Pack sizes | 20 × 10 µl or 40 × 5 µl (CYCV1501) / 80 × 10 µl or 160 × 5 µl (CYCV1502) |
| Components | pTOPO-TA Simple Vector (30 ng/µl), 1000 bp Control, 10× Enhancer |
| Storage | -20°C; stable for 12 months |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- TA cloning of Taq A-overhang inserts up to 10 kb using the Simple vector (no common flanking restriction sites).
- 5 minutes at 25–37°C; 1000 bp control insert included.
- DNA must not be 5′-phosphorylated. Competent cells are not included.
Performance at a glance
| Insert size | Up to 10 kb (design limit); amounts given for 100 bp – 5 kb (10–180 ng) |
| Insert amount guide | 100–1000 bp → 10–40 ng; 1000–2000 bp → 40–80 ng; 2000–5000 bp → 80–180 ng |
| Reaction | 5 minutes at 25–37°C (37°C recommended), 10 µl system; 10–15 minutes at 37°C for long or difficult inserts |
| Selection | Zero background — no blue-white screening needed |
| Vector | 1865 bp, high-copy, pUC ori, AmpR |
| Flanking restriction sites | No common restriction sites (simple vector) |
| Preferred insert ends | 3′-A overhang from Taq PCR products; must NOT be 5′-phosphorylated |
| Storage | −20°C, stable 12 months |
| Pack sizes | 20 × 10 µl or 40 × 5 µl (CYCV1501) / 80 × 10 µl or 160 × 5 µl (CYCV1502) |
All values from the CYCV15 manual (PDF).
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 12 months).
- DNA fragments must not be 5′-phosphorylated. TA (A-overhang) ends from Taq are preferred. Inserts up to 10 kb.
- Set up the reaction at room temperature, not on ice; incubate 5 minutes at 25–37°C (10–15 minutes at 37°C for long inserts). Mix competent cells gently — do not pipette up and down.
Who sets these specifications
Changyu Bio supplies Cat# CYCV15 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
Cat# CYCV15 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.
International ordering — CYCV15
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Shipped with ice packs; store at −20 °C on arrival.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV15 — tell us the quantity and destination city.
Alternative-product and purchasing questions
Can I choose CYCV15 instead of a TOPO TA cloning kit for PCR-product capture?
CYCV15 pTOPO-TA Simple is a TA-cloning option for A-tailed PCR products. Select it when its Simple vector design fits your downstream work; it is not an identical Invitrogen vector.
How do I choose CYCV15 Simple rather than CYCV14?
Check the two vector maps and the restriction sites needed after insert capture. Match the vector architecture, screening method and sequencing requirements to your experiment, rather than choosing only by the TA label.
How do I request a quotation for CYCV15?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Tell us the insert-end type and vector features you require.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.