CYCV09 is a PCR-based site-directed mutagenesis kit for introducing point mutations, mutations of several neighboring codons, and deletions or insertions into a target DNA (usually a plasmid). SuperPfu polymerase synthesizes the mutant plasmid, DpnI digests the methylated template, and the nicked mutant plasmid is repaired in E. coli. Mutation efficiency is generally above 90%.
Catalog no. CYCV09 · For research use only
Key features
- All steps in one PCR tube
- Partially overlapping primer design gives exponential PCR amplification with visible product on gel
- DpnI removes the methylated parent plasmid; mutation efficiency up to 90%; no special competent cells needed
- SuperPfu polymerase with 4-6 times the amplification speed of Pfu and 3 times the fidelity of Pfu
- Do not replace the supplied buffer or dNTPs; stored at -20 °C for 12 months; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV0901 | One Tube Mutagenesis Kit | 10 reactions |
| CYCV0902 | One Tube Mutagenesis Kit | 20 reactions |
Specifications
| Method | PCR mutagenesis with DpnI template removal |
|---|---|
| Mutations | Point, multi-codon, deletion, insertion |
| Efficiency | Up to and above 90% |
| Enzyme | SuperPfu DNA polymerase |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- Site-directed point mutagenesis of plasmids
- Deletion or insertion of codons
- Multi-codon mutation of neighboring sites
Ordering
International ordering — CYCV09
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Storage: -20 °C, 12 months. Shipping temperature is confirmed per item.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV09 — tell us the quantity and destination city.
One Tube Mutagenesis Kit FAQ
How does it work?
The methylated template plasmid is used for PCR with SuperPfu to make non-methylated mutant plasmid with two nicks. DpnI then digests the methylated template, and the surviving mutant plasmid is repaired in E. coli.
What mutation efficiency can I expect?
Generally above 90%.
Can I use my own buffer or dNTPs?
No. The buffer and dNTPs are specially optimized for this reaction and should not be replaced.
How do I order CYCV09?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.
Product manual
CYCV09 instructions for use (English, PDF).