2× master mix combining super-fidelity CYA9 enzyme with elongation factors for long, accurate, high-yield amplification. The better choice when both long targets and high fidelity matter.
Key Features
- Super-fidelity enzyme + elongation factors for long-range accuracy
- Greatly improved amplification length, speed, fidelity and yield
- 2× ready-to-use format: add template, primers and water
- Suitable for difficult, GC-rich and long templates
Specifications
| Format | 2× mix: CYA9 super-fidelity polymerase, dNTPs, elongation factors, optimized buffer |
| Strength | Long-range amplification with high fidelity |
| Storage | -20°C; avoid repeated freeze-thaw |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- Long PCR from complex genomic DNA (manual: less than 10 kb).
- Longer targets from simple templates (manual: less than 15 kb).
- Blunt-end products for high-fidelity cloning; red dye for direct gel loading.
Performance at a glance
| Max amplicon | Less than 10 kb on complex genomic DNA; less than 15 kb on simple gDNA or plasmid (manual) |
| Speed | 3–4 kb/min; 10–15 s/kb on simple or plasmid templates, 20–25 s/kb on complicated templates (manual) |
| Fidelity | Up to 54× of traditional Taq DNA polymerase |
| Yield | 0.5–1× of Pfu |
| Human genomic DNA evidence | Hα1AT 2.6 kb and β-globin 6 kb amplified from 50 ng human gDNA in 50 µl, extension 20 s/kb (manual, Figure 1) |
| Template input (50 µl) | Plasmid 0.1–10 ng; bacterial genome 10–100 ng; human genome 50–150 ng; cDNA 1–5 µl |
| High-GC / complex templates | Denaturation up to 98°C (no clear influence on CYA9 activity); DMSO 1–8% or betaine 1.0–1.7 M; touchdown PCR |
| Product ends | Blunt; red tracer dye for direct gel loading |
| Storage / pack sizes | −20°C, 2 years; CYPC8401 (1 ml) · CYPC8402 (5 ml) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 2 years). Avoid repeated freeze–thaw.
- Typical length: less than ~10 kb on complex genomic DNA; less than ~15 kb on simple gDNA or plasmid.
- Products are blunt-ended. High-GC templates may use 98°C denaturation as in the manual.
Who sets these specifications
Changyu Bio supplies Cat# CYPC84 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
LongHiFi FAQ
When should I choose LongHiFi instead of FastHiFi?
Both are high-fidelity mixes — pick by target length. FastHiFi (CYPC82) is the faster choice for non-long targets (≤3 kb on complex gDNA). Choose LongHiFi when length matters: the manual supports less than ~10 kb on complex genomic DNA and less than ~15 kb on simple templates.
How fast is LongHiFi?
3–4 kb/min. The manual recommends 10–15 s/kb on simple or plasmid templates and 20–25 s/kb on complicated templates. If smearing appears above and below the main band on the gel, the extension time is too long — decrease it.
Can LongHiFi handle GC-rich targets?
Yes — it is suitable for difficult, GC-rich and long templates. Raise pre-denaturation and denaturation to 98°C (no clear influence on CYA9 activity), or add DMSO 1–8% / betaine 1.0–1.7 M with touchdown PCR.
Are LongHiFi products blunt-ended?
Yes — blunt ends for high-fidelity cloning, and the mix contains a red tracer dye for direct gel loading. For long targets where maximum speed matters more than fidelity, see FastLong (CYPC80).
Cat# CYPC84 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.
Alternative-product and purchasing questions
Can CYPC84 be considered for a long-amplicon high-fidelity workflow?
CYPC84 LongHiFi is an option for high-fidelity amplification of longer targets within its manual’s scope. If you currently use Q5 or Phusion, provide the target length and template details so the CYPC84 requirements can be reviewed. Equivalent fidelity or amplification success is not assumed.
Is CYPC84 a protocol-identical replacement for another polymerase?
No. Follow the CYPC84 instructions and evaluate the target rather than carrying over another enzyme’s extension or annealing conditions unchanged. The manual describes blunt-ended products; verify the end requirements of any downstream cloning kit.
How do I request a quotation for CYPC84?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Include the instrument or cycling workflow where relevant.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.