2× master mix combining super-fidelity CYA9 enzyme with elongation factors for long, accurate, high-yield amplification. The better choice when both long targets and high fidelity matter.
Key Features
- Super-fidelity enzyme + elongation factors for long-range accuracy
- Greatly improved amplification length, speed, fidelity and yield
- 2× ready-to-use format: add template, primers and water
- Suitable for difficult, GC-rich and long templates
Specifications
| Format | 2× mix: CYA9 super-fidelity polymerase, dNTPs, elongation factors, optimized buffer |
| Strength | Long-range amplification with high fidelity |
| Storage | -20°C; avoid repeated freeze-thaw |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- Long PCR from complex genomic DNA (manual: less than 10 kb).
- Longer targets from simple templates (manual: less than 15 kb).
- Blunt-end products for high-fidelity cloning; red dye for direct gel loading.
Performance at a glance
| Max amplicon | Less than 10 kb on complex genomic DNA; less than 15 kb on simple gDNA or plasmid (manual) |
| Speed | 3–4 kb/min; 10–15 s/kb on simple or plasmid templates, 20–25 s/kb on complicated templates (manual) |
| Fidelity | Up to 54× of traditional Taq DNA polymerase |
| Yield | 0.5–1× of Pfu |
| Human genomic DNA evidence | Hα1AT 2.6 kb and β-globin 6 kb amplified from 50 ng human gDNA in 50 µl, extension 20 s/kb (manual, Figure 1) |
| Template input (50 µl) | Plasmid 0.1–10 ng; bacterial genome 10–100 ng; human genome 50–150 ng; cDNA 1–5 µl |
| High-GC / complex templates | Denaturation up to 98°C (no clear influence on CYA9 activity); DMSO 1–8% or betaine 1.0–1.7 M; touchdown PCR |
| Product ends | Blunt; red tracer dye for direct gel loading |
| Storage / pack sizes | −20°C, 2 years; CYPC8401 (1 ml) · CYPC8402 (5 ml) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 2 years). Avoid repeated freeze–thaw.
- Typical length: less than ~10 kb on complex genomic DNA; less than ~15 kb on simple gDNA or plasmid.
- Products are blunt-ended. High-GC templates may use 98°C denaturation as in the manual.
Who sets these specifications
Changyu Bio manufactures Cat# CYPC84 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
LongHiFi FAQ
When should I choose LongHiFi instead of FastHiFi?
Both are high-fidelity mixes — pick by target length. FastHiFi (CYPC82) is the faster choice for non-long targets (≤3 kb on complex gDNA). Choose LongHiFi when length matters: the manual supports less than ~10 kb on complex genomic DNA and less than ~15 kb on simple templates.
How fast is LongHiFi?
3–4 kb/min. The manual recommends 10–15 s/kb on simple or plasmid templates and 20–25 s/kb on complicated templates. If smearing appears above and below the main band on the gel, the extension time is too long — decrease it.
Can LongHiFi handle GC-rich targets?
Yes — it is suitable for difficult, GC-rich and long templates. Raise pre-denaturation and denaturation to 98°C (no clear influence on CYA9 activity), or add DMSO 1–8% / betaine 1.0–1.7 M with touchdown PCR.
Are LongHiFi products blunt-ended?
Yes — blunt ends for high-fidelity cloning, and the mix contains a red tracer dye for direct gel loading. For long targets where maximum speed matters more than fidelity, see FastLong (CYPC80).
Cat# CYPC84 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.