CYPC65 is a first-strand cDNA synthesis kit for miRNA based on the stem-loop method. Its 5x THERMO Reaction Mix is a one-tube premix with all RT components (THERMOscript H- RTase, RNase inhibitor, dNTP mixture, buffer); you add template RNA and stem-loop primers. A special gDNA Remover removes genomic DNA in the same step without opening the tube.
Catalog no. CYPC65 · For research use only
Key features
- Stem-loop miRNA reverse transcription with a one-tube 5x premix
- Premix includes THERMOscript H- RTase, RNase inhibitor, dNTP mixture and buffer
- Special gDNA Remover clears genomic DNA and runs RT in the same step, lowering contamination and RNA degradation risk
- Highly thermostable RTase and optimized buffer support specific miRNA reverse transcription
- cDNA is compatible with qPCR mixes from any company; stored at -20 °C for 12 months; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC6501 | miRNA cDNA Kit (Stem-Loop) | 50 reactions |
| CYPC6502 | miRNA cDNA Kit (Stem-Loop) | 100 reactions |
Specifications
| Method | Stem-loop RT |
|---|---|
| Premix | 5x THERMO Reaction Mix |
| Enzyme | THERMOscript H- RTase |
| gDNA removal | One-step gDNA Remover |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- miRNA reverse transcription before qPCR
- Specific miRNA detection with stem-loop primers
- Pairing with the 2x miRNA SYBR Green qPCR Mix (CYPC90)
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
miRNA cDNA Kit (Stem-Loop) FAQ
What do I add to the reaction?
Template RNA and your stem-loop primers; the 5x premix supplies the other RT components.
Do I need a separate gDNA removal step?
No. A special gDNA Remover works in the same one-step reaction.
Which qPCR mix can I use with the cDNA?
The cDNA works with qPCR mixes from any company. CYPC90 is the recommended companion.
How do I order CYPC65?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.