CYPC70 is a 5x one-tube reverse transcription premix with a one-step genomic DNA (gDNA) removal step. It uses a new-generation reverse transcriptase evolved for higher thermostability and efficiency. Reverse transcription takes 15 minutes, and it is especially suited to cDNA synthesis before two-step real-time PCR.
Catalog no. CYPC70 · For research use only
Key features
- New-generation reverse transcriptase (TRUEscript H- RTase) with much higher thermostability and efficiency
- Fully premixed: gDNA Remover, template RNA and water are added together; cDNA synthesis and gDNA removal run at once in 15 minutes
- RNA template up to 80% of total volume, suited to low-concentration RNA
- Premix does not freeze at -20 °C, reducing thaw and mixing time
- Oligo dT and N6 random primer ratio optimized for qPCR, starting cDNA synthesis along the whole transcript; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC7001 | TRUEscript RT MasterMix (OneStep gDNA Removal) | 50 reactions |
| CYPC7002 | TRUEscript RT MasterMix (OneStep gDNA Removal) | 100 reactions |
Specifications
| Format | 5x one-tube RT premix |
|---|---|
| Enzyme | TRUEscript H- RTase |
| Components | RNase inhibitor, random primers, oligo dT, dNTP mixture, buffer |
| gDNA removal | One-step gDNA Remover |
| RNA volume | Up to 80% of reaction volume |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- Two-step real-time RT-qPCR
- cDNA synthesis from low-concentration RNA
- gDNA-free reverse transcription
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
TRUEscript RT MasterMix (OneStep gDNA Removal) FAQ
Do I need DNase I treatment?
No. A gDNA Remover removes genomic DNA in the same one-step reaction, which avoids the RNA loss and extra handling of DNase I digestion.
What do I add to the reaction?
Template RNA, gDNA Remover and water; the 5x premix supplies the other RT components.
Can I use low-concentration RNA?
Yes. RNA can make up to 80% of the reaction volume, which suits low-concentration templates.
How do I order CYPC70?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.