First-strand cDNA synthesis kit using next-generation TRUEscript H− reverse transcriptase (molecular-evolution engineered, multi-site mutated) with enhanced extension ability and stability. Pre-mixed 5× TRUE Reaction Mix plus built-in gDNA Remover — one-step genomic DNA removal without extra DNase digestion.
Key Features
- TRUEscript H− RTase with enhanced extension ability and stability
- 5× TRUE Reaction Mix pre-mixed (RTase, RNase Inhibitor, dNTPs, buffer)
- One-step gDNA Remover eliminates genomic DNA contamination
- Flexible priming: Oligo(dT), Random Primer or gene-specific primer
Specifications
| Template | Total RNA 50 ng–5 µg; mRNA 5–500 ng per 20-µl reaction |
| Components | 5× TRUE Reaction Mix, gDNA Remover, RTase components, RNase-free H2O |
| Priming | Oligo(dT) / Random Primer / GSP supported |
| Storage | -20°C; avoid repeated freeze-thaw |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- First-strand cDNA synthesis (manual: up to about 12 kb) from RNA.
- Cloning or detection of high-copy and low-copy genes.
- For downstream qPCR, mix Oligo(dT) and random primer as the manual describes.
- Omit gDNA Remover when the cDNA is for gene cloning.
Performance at a glance
| cDNA length | Up to 12 kb (manual) |
| Enzyme | TRUEscript H⁻ reverse transcriptase — molecular-evolution engineered, multi-site mutated; enhanced extension and stability |
| Template | Total RNA 50 ng–5 µg; mRNA 5–500 ng per 20-µl reaction |
| RNA volume | Up to 75% of the total reaction volume |
| RT conditions | Oligo(dT)/GSP: 42°C 30–50 min (15 min for qPCR); random primer: 25°C 10 min + 42°C 30–50 min; raise to 50°C for complex secondary structure |
| Inactivation | 85°C 5 s inactivates the TRUEscript H⁻ RTase |
| Storage | −20°C ≥12 months (5× TRUE Reaction Mix does not freeze at −20°C); cDNA: −20°C up to 6 months, −70°C for long term |
| Pack sizes | CYPC1802 (50 rxns) · CYPC1803 (100 rxns) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 12 months). The 5× TRUE Reaction Mix does not freeze at −20°C.
- Do not add gDNA Remover when the cDNA will be used for gene cloning. Omit gDNA Remover if genomic DNA removal is not required.
- If the template has strong secondary structure or high GC, the manual allows raising the reverse-transcription temperature to 50°C.
Who sets these specifications
Changyu Bio manufactures Cat# CYPC1802 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
TRUEscript cDNA Kit FAQ
When should I omit the gDNA Remover?
Omit the gDNA Remover when the cDNA will be used for gene cloning, or whenever genomic DNA removal is not required. The 5× TRUE Reaction Mix and gDNA Remover are viscous — spin down briefly before use to avoid volume loss on pipette tips (manual).
How much RT product should I use as PCR template?
The manual recommends 1/10 to 1/5 of the reverse-transcription product (2–4 µl) as template for downstream PCR.
Which PCR master mixes does the manual recommend downstream?
For general amplification: CYPC80 FastLong (or CYPC09 2× Taq). For high-fidelity amplification: CYPC82 FastHiFi or CYPC84 LongHiFi.
What if my RNA has strong secondary structure?
For high-GC or complex-structured RNA, or when the cDNA will exceed 3 kb, mix the RNA, primer and RNase-free H₂O first, denature at 65°C for 5 min, cool on ice and centrifuge briefly before adding the remaining components. Alternatively raise the RT temperature to 50°C (manual).
Cat# CYPC1802 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.