First-strand cDNA synthesis kit using next-generation TRUEscript H− reverse transcriptase (molecular-evolution engineered, multi-site mutated) with enhanced extension ability and stability. Pre-mixed 5× TRUE Reaction Mix plus built-in gDNA Remover — one-step genomic DNA removal without extra DNase digestion.
Key Features
- TRUEscript H− RTase with enhanced extension ability and stability
- 5× TRUE Reaction Mix pre-mixed (RTase, RNase Inhibitor, dNTPs, buffer)
- One-step gDNA Remover eliminates genomic DNA contamination
- Flexible priming: Oligo(dT), Random Primer or gene-specific primer
Specifications
| Template | Total RNA 50 ng–5 µg; mRNA 5–500 ng per 20-µl reaction |
| Components | 5× TRUE Reaction Mix, gDNA Remover, RTase components, RNase-free H2O |
| Priming | Oligo(dT) / Random Primer / GSP supported |
| Storage | -20°C; avoid repeated freeze-thaw |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- First-strand cDNA synthesis (manual: up to about 12 kb) from RNA.
- Cloning or detection of high-copy and low-copy genes.
- For downstream qPCR, mix Oligo(dT) and random primer as the manual describes.
- Omit gDNA Remover when the cDNA is for gene cloning.
Performance at a glance
| cDNA length | Up to 12 kb (manual) |
| Enzyme | TRUEscript H⁻ reverse transcriptase — molecular-evolution engineered, multi-site mutated; enhanced extension and stability |
| Template | Total RNA 50 ng–5 µg; mRNA 5–500 ng per 20-µl reaction |
| RNA volume | Up to 75% of the total reaction volume |
| RT conditions | Oligo(dT)/GSP: 42°C 30–50 min (15 min for qPCR); random primer: 25°C 10 min + 42°C 30–50 min; raise to 50°C for complex secondary structure |
| Inactivation | 85°C 5 s inactivates the TRUEscript H⁻ RTase |
| Storage | −20°C ≥12 months (5× TRUE Reaction Mix does not freeze at −20°C); cDNA: −20°C up to 6 months, −70°C for long term |
| Pack sizes | CYPC1802 (50 rxns) · CYPC1803 (100 rxns) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 12 months). The 5× TRUE Reaction Mix does not freeze at −20°C.
- Do not add gDNA Remover when the cDNA will be used for gene cloning. Omit gDNA Remover if genomic DNA removal is not required.
- If the template has strong secondary structure or high GC, the manual allows raising the reverse-transcription temperature to 50°C.
Who sets these specifications
Changyu Bio supplies Cat# CYPC1802 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
TRUEscript cDNA Kit FAQ
When should I omit the gDNA Remover?
Omit the gDNA Remover when the cDNA will be used for gene cloning, or whenever genomic DNA removal is not required. The 5× TRUE Reaction Mix and gDNA Remover are viscous — spin down briefly before use to avoid volume loss on pipette tips (manual).
How much RT product should I use as PCR template?
The manual recommends 1/10 to 1/5 of the reverse-transcription product (2–4 µl) as template for downstream PCR.
Which PCR master mixes does the manual recommend downstream?
For general amplification: CYPC80 FastLong (or CYPC09 2× Taq). For high-fidelity amplification: CYPC82 FastHiFi or CYPC84 LongHiFi.
What if my RNA has strong secondary structure?
For high-GC or complex-structured RNA, or when the cDNA will exceed 3 kb, mix the RNA, primer and RNase-free H₂O first, denature at 65°C for 5 min, cool on ice and centrifuge briefly before adding the remaining components. Alternatively raise the RT temperature to 50°C (manual).
Cat# CYPC1802 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.
Alternative-product and purchasing questions
Is CYPC1802 an alternative to RevertAid K1622 for first-strand cDNA synthesis?
CYPC1802 TRUEscript is an option for first-strand cDNA preparation from RNA. Its kit includes a gDNA-removal component. Review the supplied primers, reaction format and input requirements; it is not an identical RevertAid formulation.
Is this kit a one-step RT-qPCR master mix?
No. CYPC1802 prepares first-strand cDNA for subsequent analysis. The PCR or qPCR reagents are a separate step. Follow its own gDNA-removal and reverse-transcription instructions rather than transferring a K1622 protocol unchanged.
How do I request a quotation for CYPC1802?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Include the instrument or cycling workflow where relevant.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.