CYPC01 is Taq DNA Polymerase, purified from E. coli expressing the Thermus aquaticus polymerase gene, supplied with a buffer that includes Mg2+. The enzyme is 94 kDa with 5′-3′ polymerase and 5′-3′ exonuclease activity and no 3′-5′ exonuclease activity. Extension speed is 1-2 kb/min, and products have a 3′ A overhang.
Catalog no. CYPC01 · For research use only
Key features
- Molecular weight 94 kDa; 5′-3′ polymerase and 5′-3′ exonuclease activity, no 3′-5′ exonuclease activity
- Extension speed of 1-2 kb/min
- Products carry a 3′ A overhang for T/A cloning
- Used for PCR, DNA labelling, primer extension, sequencing and adding A to blunt ends
- Supplied at 5 U/μL with Mg2+ in the buffer; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC0102 | Taq DNA Polymerase (Buffer With Mg2+) | 500 U |
| CYPC0103 | Taq DNA Polymerase (Buffer With Mg2+) | 1000 U |
| CYPC0104 | Taq DNA Polymerase (Buffer With Mg2+) | 3000 U |
Specifications
| Enzyme | Taq DNA Polymerase |
|---|---|
| Concentration | 5 U/μL |
| Molecular weight | 94 kDa |
| Extension speed | 1-2 kb/min |
| Buffer | Contains Mg2+ |
| Storage | -20 °C |
| Use | Research use only |
Applications
- PCR amplification of DNA fragments
- DNA labelling and primer extension
- Sequencing templates
- Adding A to blunt ends
- T/A cloning
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Taq DNA Polymerase (Buffer With Mg2+) FAQ
Does it have proofreading activity?
No. It has 5′-3′ polymerase and 5′-3′ exonuclease activity and no 3′-5′ exonuclease activity.
Can products be used for T/A cloning?
Yes. Products have a 3′ A overhang.
Does the buffer contain magnesium?
Yes, the supplied buffer includes Mg2+.
How do I order CYPC01?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
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