Ready-to-use 2×SYBR Green qPCR Mix for rapid, sensitive real-time quantification of DNA targets. The mix combines HotMaster Taq DNA Polymerase (hot-start), dNTPs, optimized buffer, BSA and SYBR Green I — no probe synthesis needed for most targets.
Key Features
- Hot-start HotMaster Taq DNA Polymerase blocks mis-primed products and primer-dimers
- SYBR Green I dye detects many targets without target-specific probes
- High specificity, sensitivity and accurate quantification in real-time PCR
- Convenient 2× format — just add template, primers and water
Specifications
| Pack sizes | 50 rxns (CYPC3301) / 200 rxns (CYPC3302) |
| Format | 2× mix; 1.25 ml per 50-µl reaction × 50 reactions, or 4×1.25 ml × 200 reactions |
| Components | 2×SYBR qPCR Mix (HotMaster Taq, dNTPs, buffer, BSA, SYBR Green I) |
| Storage | -20°C long term (protect from light), 4°C short term; avoid repeated freeze-thaw |
| Shipping | Ice packs; store at the listed temperature on arrival |
| Note | ROX reference dye not included — select per your qPCR instrument protocol |
Typical Applications
- Real-time quantification of DNA targets with SYBR Green I (no probe synthesis).
- Research qPCR when amplicons are about 100–150 bp; the manual says products should not exceed 500 bp.
- Confirm products by melting curve or gel — this is a dye assay, not a TaqMan-style probe mix.
- Add ROX (CYPC38) only if the instrument table requires it; it is not in this mix.
Performance at a glance
| Amplicon range | Optimal 100–150 bp; PCR products should not exceed 500 bp (manual) |
| Enzyme | Hot-start HotMaster Taq DNA Polymerase — blocks mis-primed products and primer-dimers |
| Mg²⁺ | 4 mM in the mix; recommended initial concentration 2 mM; adjust with 25 mM MgCl₂ |
| Cycling | Two-step: 94°C 5–10 s / 60°C 30–34 s; three-step: 94°C 10–20 s / 55–60°C 10–20 s / 72°C 20–30 s; 40 cycles (manual) |
| Instrument evidence | Amplification and melting curves on Roche LightCycler 480 II with three-step method (manual, Typical Data) |
| ROX | Not included — add CYPC38 only if your instrument protocol requires it |
| Storage | −20°C protected from light for more than 6 months; 4°C for short-term use; SYBR Green I is light-sensitive |
| Pack sizes | CYPC3301 (50 rxns, 1.25 ml) · CYPC3302 (200 rxns, 4 × 1.25 ml) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Protect from light (SYBR Green I). Avoid repeated freeze–thaw; store at −20°C long term, 4°C short term.
- ROX reference dye is not included — add CYPC38 only if your instrument requires it.
- This is a dye-based SYBR Green mix, not a probe (TaqMan-style) assay mix.
Who sets these specifications
Changyu Bio manufactures Cat# CYPC33 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
SYBR qPCR Mix FAQ
Is this a probe-based (TaqMan-style) assay mix?
No. This is a dye-based SYBR Green I mix — no probe synthesis is needed for most targets. Because it is a dye assay, confirm products by melting curve or gel.
Do I need to add ROX?
ROX is not included in the mix. ABI- or Stratagene-type instruments that require passive reference normalization should add CYPC38 ROX Reference Dye per the instrument protocol; Roche, Bio-Rad and Eppendorf cyclers typically need no ROX.
Two-step or three-step cycling?
The manual gives both. Two-step (94°C 5–10 s / 60°C 30–34 s) has higher specificity; three-step (94°C 10–20 s / 55–60°C 10–20 s / 72°C 20–30 s) has higher efficiency. If the melting curve is poor, use the two-step method; if primer Tm is too low, extend the extension time or switch to three-step.
What amplicon size does the manual recommend?
For optimal results, PCR products should be between 100 and 150 bp, and should not exceed 500 bp. Prepare the PCR reaction on ice for higher specificity and less background.
Cat# CYPC33 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.