ROX Reference Dye for real-time PCR instruments that require passive reference normalization (e.g. ABI, Stratagene). Supplied at 50 µmol (100×) stock; final reaction concentration typically 500 nmol (1×) per instrument protocol.
Key Features
- Normalizes well-to-well signal differences from pipetting error
- Compatible with ABI and Stratagene real-time instruments
- Simple 100× stock — dilute per your instrument’s protocol
- Small pack sizes ideal for evaluation (25 µl / 100 µl)
Specifications
| Pack sizes | 25 µl (CYPC3801) / 100 µl (CYPC3802) |
| Concentration | 50 µmol (100×) stock; working concentration ~500 nmol (1×) |
| Storage | -20°C; stable for at least 12 months |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- Well-to-well fluorescence normalization on ABI- or Stratagene-type real-time instruments.
- High-ROX instruments (1×) vs low-ROX (0.1×) — volumes are in the product table.
- Do not add ROX on Roche, Bio-Rad, or Eppendorf cyclers (manual: not needed).
Performance at a glance
| Stock | 50 µmol (100×) |
| Final concentration | 500 nmol (1×, high) or 50 nmol (0.1×, low) depending on instrument (manual) |
| Instruments — 1× (0.5 µl per 50-µl reaction) | ABI PRISM 7000/7300/7700/7900HT, ABI StepOne / StepOne Plus |
| Instruments — 0.1× (0.05 µl per 50-µl reaction) | ABI 7500/7500 Fast, Stratagene Mx3000P/Mx3005P/Mx4000, MJ Research Chromo4/Opticon, Corbett Rotor-Gene 3000 |
| No ROX needed | Roche, Bio-Rad, Eppendorf cyclers (manual) |
| Quick use | High-ROX: 25 µl per 1.25 ml 2× SYBR qPCR Mix; low-ROX: 2.5 µl per 1.25 ml; subtract ROX volume from ddH₂O |
| Small additions | Dilute the dye 10–20× in water before adding 0.05 µl so the volume is pipettable |
| Storage / pack sizes | −20°C, at least 12 months; CYPC3801 (25 µl) · CYPC3802 (100 µl) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: at least 12 months).
- Roche, Bio-Rad, and Eppendorf cyclers typically do not need ROX. ABI 7000/7300/7700/7900/StepOne use 1×; ABI 7500 and several Stratagene/MJ/Corbett models use 0.1×.
- For 0.05 µl additions, dilute the dye 10–20× in water first so the volume is pipettable.
Who sets these specifications
Changyu Bio manufactures Cat# CYPC38 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
ROX Reference Dye FAQ
Which instruments need ROX?
ABI and Stratagene-type instruments that use passive reference normalization — see the instrument table above for exact 1× vs 0.1× requirements. Roche, Bio-Rad and Eppendorf cyclers typically do not need ROX (manual).
How do I add ROX to my qPCR mix?
For high-ROX instruments (1×), add 25 µl ROX per 1.25 ml of 2× SYBR qPCR Mix (CYPC33); for low-ROX (0.1×), add 2.5 µl. Mix well and use directly. Subtract the ROX volume from the ddH₂O in the reaction.
What if my reaction needs only 0.05 µl of ROX?
Dilute the ROX Reference Dye 10–20× in water first, so the volume you pipette is accurate and reproducible (manual).
Cat# CYPC38 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.