One-step RT-PCR kit that performs reverse transcription and PCR amplification in a single tube, using mutation-optimized TRUEscript reverse transcriptase. No tube opening between steps — reducing contamination risk and improving sensitivity.
Key Features
- RT and PCR in one tube — simple workflow, lower contamination risk
- Mutation-modified TRUEscript RTase with enhanced sensitivity and efficiency
- Optimized OneStep Enzyme Mix pre-mixed at the right ratio
- Accepts 1 pg–1 µg RNA template per 20-µl reaction
Specifications
| Pack sizes | 125 rxns × 20-µl system (CYPC3401) / 250 rxns × 20-µl system (CYPC3402) |
| Components | 2×RT-PCR Mix, TRUEscript OneStep Enzyme Mix, RNase-free H2O |
| Storage | -20°C; stable for 6 months |
| Shipping | Ice packs; store at the listed temperature on arrival |
Typical Applications
- One-tube reverse transcription plus PCR from RNA (no separate cDNA kit).
- High-copy or low-copy transcript detection, including high-GC or structured RNA templates.
- Do not use Oligo(dT) or random primers with this kit — that workflow is CYPC1802.
Performance at a glance
| Speed | PCR extension 1–2 kb/min (manual) |
| Enzyme system | Mutation-modified TRUEscript M-MuLV H⁻ reverse transcriptase + hot-start HotMaster Taq + RNasin inhibitor mix |
| Template | RNA 1 pg–1 µg per 20-µl reaction (adjust per target — too much RNA can inhibit) |
| Reverse transcription | 42°C standard, up to 50°C for high-GC or structured RNA; preheat the cycler to 42°C before loading (manual) |
| Cycling | 42°C 30 min → 94°C 2–3 min → 94°C 30 s / 50–60°C 30 s / 72°C 1–2 kb/min × 30–40 → 72°C 5–10 min |
| Priming | Gene-specific primers only — no oligo(dT) or random primers (that workflow is CYPC1802) |
| Storage | −20°C, 6 months; 4°C for short-term frequent use; thaw and mix gently — avoid bubbles |
| Shipping / pack sizes | Blue ice; CYPC3401 (125 rxns × 20 µl) · CYPC3402 (250 rxns × 20 µl) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped with ice packs. Store at the temperature in the specifications table on arrival.
- Store at −20°C (manual: 6 months). Mix may be kept at 4°C for short-term frequent use.
- One-step RT-PCR uses gene-specific primers. Do not use oligo(dT) or random primers with this kit.
- Thaw and mix the 2× RT-PCR Mix gently — avoid violent swirling that introduces bubbles.
Who sets these specifications
Changyu Bio manufactures Cat# CYPC34 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
TRUEscript One-Step FAQ
Can I use oligo(dT) or random primers with this kit?
No. One-step RT-PCR uses gene-specific primers; oligo(dT) and random primers cannot be used for cDNA synthesis with this kit. That workflow is covered by the TRUEscript 1st Strand cDNA Synthesis Kit (CYPC1802).
How much RNA template per reaction?
1 pg–1 µg per 20-µl reaction. Different fragments need different optimal amounts and too much RNA can inhibit the reaction — adjust according to the actual amplification (manual).
What temperature should the reverse transcription run at?
42°C is standard. For templates with strong secondary structure or high GC, the reverse transcription temperature can be raised to 50°C. Preheat the PCR cycler to 42°C before placing the tubes in (manual).
How does the one-tube workflow help?
Reverse transcription and PCR run in a single tube with no opening between steps, reducing contamination risk and improving sensitivity. Keep cycle number to the minimum that gives sufficient yield — too many cycles raise mismatch and background.
Cat# CYPC34 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.