CYPC39 is a 2x premixed reagent for real-time PCR using probe hybridization. It contains DNA polymerase, dNTPs, a stabilizer and optimized buffer, and uses HotMaster Taq DNA polymerase to suppress nonspecific amplification for high-sensitivity reactions. It is used by adding template, primers and probe with water.
Catalog no. CYPC39 · For research use only
Key features
- Premixed 2x format for probe-based real-time PCR
- HotMaster Taq hot-start polymerase effectively suppresses nonspecific amplification
- Described as sensitive, specific and stable
- Temperature-dependent inhibitor block keeps the enzyme inactive below 40 °C
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC3901 | 2x Real Time PCR Mix (Probe) | 100 reactions (20 μL) |
| CYPC3902 | 2x Real Time PCR Mix (Probe) | 500 reactions (20 μL) |
Specifications
| Format | 2x premix, probe detection |
|---|---|
| Enzyme | HotMaster Taq DNA polymerase (hot start) |
| Components | Polymerase, dNTPs, stabilizer, buffer |
| Storage | -20 °C, protected from light, at least 6 months |
| Working storage | 4 °C short term; avoid repeated freeze-thaw |
| Use | Research use only |
Applications
- Probe-based real-time PCR
- Sensitive target detection by qPCR
- Gene quantification assays
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
2x Real Time PCR Mix (Probe) FAQ
What detection chemistry is it for?
Probe hybridization real-time PCR.
How does the hot start work?
HotMaster Taq DNA polymerase blocks the substrate-binding site with an inhibitory ligand in a temperature-dependent way: below 40 °C an inactive enzyme-inhibitor complex forms, and at the primer annealing temperature the equilibrium shifts toward the template-primer complex.
How is it stored?
At -20 °C protected from light for at least 6 months. Thaw and mix fully before use; short-term use can be at 4 °C. Avoid repeated freeze-thaw.
How do I order CYPC39?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.