CYPC48 is a miRNA first-strand cDNA synthesis kit based on poly(A) tailing. miRNA 3′ ends are first tailed with poly(A), then reverse transcribed with an anchored oligo(dT)-universal tag primer to make cDNA. A special premix performs tailing and reverse transcription in one step, and it must be used with the miRNA qPCR detection kit CYPC49.
Catalog no. CYPC48 · For research use only
Key features
- Poly(A) tailing and reverse transcription combined in one step
- Anchored oligo(dT)-universal tag primer
- Works with 20 pg to 2 μg of total RNA
- One cDNA synthesis can be used to detect several microRNAs
- Must be used with CYPC49; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC4801 | Enhanced miRNA First-Strand Synthesis Kit | 25 reactions |
Specifications
| Method | Poly(A) tailing, then anchored oligo(dT) RT |
|---|---|
| RNA input | 20 pg – 2 μg total RNA |
| Pairs with | CYPC49 miRNA qPCR detection kit |
| Storage | -20 °C |
| Use | Research use only |
Applications
- First-strand cDNA for miRNA qPCR
- Profiling several miRNAs from one cDNA
- Low-input miRNA analysis
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Enhanced miRNA First-Strand Synthesis Kit FAQ
What RNA input does it accept?
From 20 pg to 2 μg of total RNA.
What do I use for detection?
It must be used together with the miRNA qPCR detection kit CYPC49.
How does the method work?
miRNA 3′ ends get a poly(A) tail, then an anchored oligo(dT)-universal tag primer drives reverse transcription to produce first-strand cDNA.
How do I order CYPC48?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.