CYPC46 is a two-step SYBR Green qRT-PCR kit that combines the thermostable THERMOscript 1st Strand cDNA Synthesis Kit (CYPC44) with 2x SYBR Green qPCR Mix (CYPC33). cDNA is first made with the first-strand kit, then used as template for fluorescent quantitative PCR. Follow the instructions of both component kits.
Catalog no. CYPC46 · For research use only
Key features
- Combines CYPC44 (thermostable first-strand cDNA synthesis) and CYPC33 (2x SYBR Green qPCR Mix)
- Two-step workflow: cDNA synthesis, then SYBR Green qPCR
- Supplied for 50 μL reactions
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC4601 | THERMOscript SYBR Green qRT-PCR Kit (Two-Step) | 25 reactions (50 μL) |
| CYPC4602 | THERMOscript SYBR Green qRT-PCR Kit (Two-Step) | 50 reactions (50 μL) |
Specifications
| Format | Two-kit combination |
|---|---|
| Components | CYPC44 and CYPC33 |
| Workflow | Two-step RT then qPCR |
| Reaction volume | 50 μL |
| Storage | -20 °C |
| Use | Research use only |
Applications
- Two-step qRT-PCR gene expression analysis
- SYBR Green I quantification of cDNA
- Convenient combined purchase of RT and qPCR reagents
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
THERMOscript SYBR Green qRT-PCR Kit (Two-Step) FAQ
What is in the kit?
The thermostable THERMOscript 1st Strand cDNA Synthesis Kit (CYPC44) and 2x SYBR Green qPCR Mix (CYPC33).
How is the workflow done?
First synthesize cDNA with the first-strand kit, then use the cDNA as template for SYBR Green qPCR. Follow the instructions of both component kits.
How is it stored?
At -20 °C.
How do I order CYPC46?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.