CYPC17 is an RNase H-minus M-MuLV reverse transcriptase that synthesizes first-strand cDNA from RNA templates. It is a recombinant point-mutant enzyme supplied at 200 units/μL.
Catalog no. CYPC17 · For research use only
Key features
- Recombinant point-mutant M-MuLV reverse transcriptase lacking RNase H activity
- Wild-type M-MuLV RNase H can degrade the RNA strand of RNA/DNA hybrids during first-strand synthesis; this enzyme avoids that
- Stronger extension ability and stability than wild-type M-MuLV
- Suited to long cDNA synthesis and construction of high-proportion full-length cDNA libraries
- Supplied at 200 units/μL; for research use only.
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC1702 | TRUEscript H Minus M-MuLV Reverse Transcriptase | 10,000 U |
| CYPC1703 | TRUEscript H Minus M-MuLV Reverse Transcriptase | 10,000 U x 5 tubes |
Specifications
| Enzyme | M-MuLV (RNase H-minus) reverse transcriptase |
|---|---|
| Concentration | 200 units/μL |
| Origin | Recombinant, point-mutated |
| Storage | -20 °C |
| Use | Research use only |
Applications
- First-strand cDNA synthesis
- Long cDNA synthesis
- Full-length cDNA library construction
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
TRUEscript H Minus M-MuLV Reverse Transcriptase FAQ
What does RNase H-minus mean for this enzyme?
The RNase H activity that degrades RNA in RNA/DNA hybrids has been removed, so the template RNA is not degraded during first-strand synthesis.
What is the enzyme concentration?
200 units/μL.
How should it be stored?
Store at -20 °C.
How do I order CYPC17?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
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