CYPC05 is HotMaster Taq DNA Polymerase, a hot-start enzyme that uses a synthetic affinity ligand to block the enzyme reversibly in a temperature-dependent way. It needs no extra heat-activation step and the reaction contains no denatured antibody or other protein. Products have a 3′ A overhang and can be cloned into TA vectors.
Catalog no. CYPC05 · For research use only
Key features
- Fast: no separate heat-activation step required
- Enzyme activity stays controlled during annealing, giving a hot-start effect throughout the run
- No denatured antibody or other protein contamination in the PCR
- Products have a 3′ A overhang for TA vector cloning
- Supplied at 5 U/μL; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC0502 | HotMaster Taq DNA Polymerase (Hot-Start) | 500 U |
| CYPC0503 | HotMaster Taq DNA Polymerase (Hot-Start) | 2500 U |
Specifications
| Enzyme | HotMaster Taq DNA polymerase |
|---|---|
| Hot-start mechanism | Temperature-dependent ligand block |
| Concentration | 5 U/μL |
| Product ends | 3′ A overhang |
| Storage | -20 °C |
| Use | Research use only |
Applications
- Specific PCR with reduced nonspecific products
- TA cloning of PCR products
- Routine hot-start PCR
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
HotMaster Taq DNA Polymerase (Hot-Start) FAQ
How does the hot start work?
HotMaster Taq DNA polymerase blocks the substrate-binding site with an inhibitory ligand in a temperature-dependent way: below 40 °C an inactive enzyme-inhibitor complex forms, and at the primer annealing temperature the equilibrium shifts toward the template-primer complex.
Does it need a separate activation step?
No. The product description lists fast operation with no extra heat activation.
Can I clone the PCR product into TA vectors?
Yes. Products have a 3′ A overhang.
How do I order CYPC05?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.