CYPC88 is a 5x one-tube reverse transcription premix with a one-step genomic DNA (gDNA) removal step. It uses a high-temperature reverse transcriptase (THERMOscript H- RTase) that works up to 60 °C and can read through GC-rich RNA with complex secondary structure. Reverse transcription takes 5-15 minutes, and it suits cDNA synthesis for real-time PCR or cloning-fragment amplification.
Catalog no. CYPC88 · For research use only
Key features
- High-temperature reverse transcriptase working up to 60 °C, reading through GC-rich, structured RNA
- CT values generally 2-3 cycles earlier
- Fully premixed: add RNA and water only, with gDNA Remover; reverse transcription in 5-15 minutes
- RNA template up to 80% of total volume, suited to low-concentration RNA
- Premix does not freeze at -20 °C; oligo dT and N6 ratio optimized for qPCR; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC8801 | THERMOscript RT MasterMix (OneStep gDNA Removal) | 50 reactions |
| CYPC8802 | THERMOscript RT MasterMix (OneStep gDNA Removal) | 100 reactions |
Specifications
| Format | 5x one-tube RT premix |
|---|---|
| Enzyme | THERMOscript H- RTase (up to 60 °C) |
| Components | RNase inhibitor, random primers, oligo dT, dNTP mixture, buffer |
| gDNA removal | One-step gDNA Remover |
| RNA volume | Up to 80% of reaction volume |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- Reverse transcription of GC-rich, structured RNA
- cDNA synthesis for real-time PCR
- cDNA for amplifying fragments to clone
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
THERMOscript RT MasterMix (OneStep gDNA Removal) FAQ
Do I need DNase I treatment?
No. A gDNA Remover removes genomic DNA in the same one-step reaction, which avoids the RNA loss and extra handling of DNase I digestion.
What do I add to the reaction?
Template RNA, gDNA Remover and water; the 5x premix supplies the other RT components.
Can I use low-concentration RNA?
Yes. RNA can make up to 80% of the reaction volume, which suits low-concentration templates.
How do I order CYPC88?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.