CYPC41 is a one-step qRT-PCR kit based on SYBR Green I intercalating fluorescence for real-time detection. Reverse transcription and amplification run in a single tube with real-time monitoring.
Catalog no. CYPC41 · For research use only
Key features
- OneStep Enzyme Mix of mutated TRUEscript M-MuLV H Minus RTase, hot-start HotMaster Taq and RNasin inhibitor
- 2x qRT-PCR SYBR Green Mix for RT and fluorescent PCR
- RT at 42-50 °C with improved efficiency on GC-rich, structured templates
- Hot-start polymerase reduces non-specific products
- Suited to trace RNA, including RNA viruses; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC4101 | TRUEscript One Step qRT-PCR SYBR Green Kit | 100 reactions x 20 μL |
| CYPC4102 | TRUEscript One Step qRT-PCR SYBR Green Kit | 200 reactions x 20 μL |
Specifications
| Format | One-step qRT-PCR kit, SYBR Green I |
|---|---|
| Reaction size | 20 μL |
| RT temperature | 42-50 °C |
| Components | OneStep Enzyme Mix, 2x qRT-PCR SYBR Green Mix |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- One-step qRT-PCR
- Trace RNA and RNA virus detection
- Target gene quantification
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
TRUEscript One Step qRT-PCR SYBR Green Kit FAQ
What detection chemistry is used?
SYBR Green I intercalating fluorescence.
What reduces non-specific amplification?
The hot-start HotMaster Taq DNA polymerase.
What RT temperature applies?
42-50 °C.
How do I order CYPC41?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.