CYPC68 is a one-tube 5x high-temperature reverse transcription premix for cDNA synthesis ahead of qPCR. Its enzyme works at up to 60 °C and reads through GC-rich RNA with complex secondary structure.
Catalog no. CYPC68 · For research use only
Key features
- Premix includes the components for genomic DNA removal and RNA reverse transcription; add only RNA and water
- Reverse transcription completed in 5 minutes
- Ct values are reported to come 2-3 cycles earlier in general
- RNA template up to 80% of total volume
- Compatible with dye-based and probe-based qPCR, or amplification of cloning fragments
- Research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC6801 | THERMOscript RT MasterMix (For Real Time PCR) | 50 reactions x 20 μL |
| CYPC6802 | THERMOscript RT MasterMix (For Real Time PCR) | 100 reactions x 20 μL |
Specifications
| Format | 5x premix, one tube |
|---|---|
| Reaction size | 20 μL |
| Temperature | Up to 60 °C |
| RT time | 5 minutes |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- cDNA synthesis for qPCR (dye or probe)
- Amplification of cloning fragments
- Reverse transcription of GC-rich RNA
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
THERMOscript RT MasterMix (For Real Time PCR) FAQ
Which qPCR chemistries can follow it?
Both dye-based and probe-based qPCR.
How long is the RT reaction?
About 5 minutes.
How much RNA can be added?
Up to 80% of the total volume.
How do I order CYPC68?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.