CYPC03 is SuperPfu DNA Polymerase, a genetically engineered Pyrococcus furiosus enzyme with 5′-3′ polymerase and 3′-5′ exonuclease activity. The proofreading activity corrects mismatched bases during amplification, making it suited to high-fidelity PCR. Products are blunt-ended.
Catalog no. CYPC03 · For research use only
Key features
- Engineered from Pyrococcus furiosus DNA polymerase for higher activity, stability and extension speed
- 5′-3′ polymerase activity and 3′-5′ exonuclease (proofreading) activity
- Described as having an extremely low error rate among thermostable DNA polymerases
- Blunt-end products can be cloned directly into blunt vectors
- Supplied at 5 U/μL; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC0302 | SuperPfu DNA Polymerase | 500 U |
| CYPC0303 | SuperPfu DNA Polymerase | 3000 U |
Specifications
| Enzyme | SuperPfu DNA Polymerase |
|---|---|
| Concentration | 5 U/μL |
| Activities | 5′-3′ polymerase; 3′-5′ exonuclease |
| Product ends | Blunt |
| Storage | -20 °C |
| Use | Research use only |
Applications
- High-fidelity DNA amplification
- Gene expression cloning
- Site-directed mutagenesis
- SNP analysis in cells
- Blunt-end fill-in
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
SuperPfu DNA Polymerase FAQ
Does it have proofreading activity?
Yes. It has 3′-5′ exonuclease activity that corrects mismatched bases during amplification.
Can the PCR product be cloned directly?
The products are blunt-ended and can be used directly with blunt-end vectors.
What concentration is supplied?
5 U/μL, stored at -20 °C.
How do I order CYPC03?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.