Changyu Bio supplies empty silica membrane spin columns in two formats, Mini and Micro, each packed with 2 mL collection tubes. They are for labs and kit developers who prepare their own lysis, wash and elution buffers for DNA or RNA purification. Changyu Bio has supplied these columns for nearly ten years. Research Use Only.
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Specifications
| Mini spin column | Micro spin column | |
|---|---|---|
| Catalog number | CYADL-2ML | CYADL-5UL |
| Membrane | Silica membrane; 6-layer and 8-layer versions available | |
| Collection tube | 2 mL collection tube included | |
| Pack size | 50 columns per pack | |
| Dimensions | Standard universal spin column dimensions, cross-compatible with common 2 mL tubes and microcentrifuges | |
| Minimum order | Single-unit orders accepted, subject to confirmation; bulk quantities quoted on request | |
| Maximum loading volume per spin | ~700 µL | ~700 µL |
| Typical binding capacity | Up to ~100 µg nucleic acid (standard mini format) | Up to ~5 µg (standard micro format, for small samples) |
| Minimum elution volume | ~30 µL | 5 µL |
| Note | Binding capacity and recovery depend on your lysis/binding buffer and sample type; values follow the standard mini and micro spin column formats used worldwide. | |
Mini or Micro: which one?
Mini columns are the general-purpose format used in most plasmid, genomic DNA, PCR clean-up, gel extraction and total RNA protocols. Micro columns elute in as little as 5 µL, for a concentrated eluate from small samples such as sorted cells, biopsies or low-input libraries. Tell us your sample type and expected yield and we will recommend a format.
6-layer or 8-layer membrane?
More silica membrane layers give more binding surface. Tell us your sample input and expected nucleic acid yield when you request a quote, and we will confirm which version fits your protocol.
How silica spin columns work
Nucleic acid binds the silica membrane in the presence of chaotropic salt and ethanol, contaminants are removed with ethanol-based wash buffers, and DNA or RNA is released in a low-salt elution buffer. Full explanation and troubleshooting: silica spin column chemistry.
Spin column for DNA or RNA extraction: empty column or complete kit?
These are empty silica membrane spin columns with 2 mL collection tubes. Choose them if you already have your own lysis, binding and wash buffers. If you want a validated workflow for a specific sample, pick the matching kit below; it contains the columns and every buffer.
| What you need | Best choice |
|---|---|
| Columns for your own DNA or RNA protocol | These spin columns: Mini CYADL-2ML or Micro CYADL-5UL, 6- or 8-layer membrane |
| Genomic DNA from whole blood | CYDN01 Blood Genomic DNA Mini Kit |
| Genomic DNA from cells, tissue or mouse tail | CYDN07 Plus DNA Mini Kit |
| Genomic DNA from blood, tissue, bacteria or insects | CYDN10 Universal DNA Extraction Kit |
| Total RNA | CYRN03 RNApure Total RNA Kit or CYRN28 Plus RNA Mini Kit |
| RNA from polysaccharide- or polyphenol-rich plants | CYRN53 Plus Complex Plant RNA Mini Kit |
| PCR product cleanup / DNA from agarose gel | CYDR02 PCR Purification Kit / CYDR01 Gel Extraction Kit |
Not sure how binding works on silica? Read silica spin column chemistry: how DNA and RNA bind, wash and elute.
Prefer a complete kit?
If you would rather not prepare buffers yourself, Changyu Bio also supplies complete spin column kits such as the CYRN28 Plus RNA Mini Kit, CYDR02 PCR Purification Kit and CYDR01 Gel Extraction Kit. See all RNA/DNA extraction kits.
Request a quote
Send the catalog number or format (Mini CYADL-2ML or Micro CYADL-5UL), membrane (6-layer or 8-layer), quantity and destination country. Changyu Bio replies with lead time and a commercial quotation; small test orders are welcome.
Common questions
- Are collection tubes included? Yes, each column comes with a 2 mL collection tube.
- Will they fit my centrifuge and rack? They use standard universal spin column dimensions and are cross-compatible with most systems.
- Is there a minimum order? No. Order any quantity; freight is charged at cost. Larger orders receive bulk pricing.
- Do you supply buffers? Columns are sold empty. For a ready-to-use workflow, choose one of the complete kits above.
Can I use these columns for protein purification or buffer exchange?
No. Silica membranes bind nucleic acids under high-salt (chaotropic) conditions; they are not designed for protein purification, desalting or buffer exchange. Use a resin, desalting or ultrafiltration column for proteins.
For laboratory research use only. Not for diagnostic or therapeutic use.