Combines TRIzol®-grade lysis (Buffer RL) with silica spin-column purification to isolate ultrapure total RNA from animal and plant cells and tissues in under an hour. Superior lysis power with a convenient, time-saving protocol.
Key Features
- TRIzol®-based lysis + spin-column purification
- Superior lysis capability for cells and tissues
- Ultrapure total RNA in under one hour
- TRIzol-style lysis with chloroform phase separation, then spin-column purification; no alcohol precipitation
Specifications
| Pack size | 50 Preps (CYRN0302) |
| Components | Buffer RL (TRIzol® Reagent), Wash Buffer RE, Wash Buffer RW, RNase-free H2O, RNA Bind Columns |
| Storage | Buffer RL at 4°C; other reagents at RT; stable for 9 months |
| Shipping | Ambient (room temperature); no ice packs |
| Note | Add indicated ethanol to Wash Buffer RW before first use |
Typical Applications
- Total RNA from animal or plant tissue, or cultured cells, using Buffer RL (TRIzol) plus a spin column.
- Aqueous-phase RNA after chloroform separation.
- Optional on-column DNase if the downstream step needs DNA-free RNA.
Input ranges at a glance
| Tissues | 50–100 mg per 1 ml Buffer RL; sample volume must not exceed 10% of the Buffer RL volume |
| Adherent cells | Lyse directly in the dish: 1 ml Buffer RL per 10 cm² (do not wash cells before adding Buffer RL) |
| Suspension cells | 5–10 × 10⁶ animal/plant/yeast cells or 1 × 10⁷ bacterial cells per 1 ml Buffer RL |
| Aqueous phase | About 500–600 µl after centrifugation; transfer ~480 µl |
| Ethanol | 0.5 volume (240 µl) — 33% final concentration |
| Elution | 30–50 µl RNase-free water; 70–90°C pre-warming raises yield |
| Storage / pack sizes | Buffer RL (TRIzol) at 4°C, other components RT; stable 9 months; CYRN0302 (50 preps) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped at ambient temperature (no ice packs).
- Buffer RL at 4°C; other reagents at room temperature. Stable 9 months at the indicated temperatures.
- Sample volume should not exceed 10% of Buffer RL. Vortexing can increase DNA contamination of RNA.
- Purified RNA: on ice if used within hours; −80°C for long-term storage. On-column DNase is a separate kit (not included).
Who sets these specifications
Changyu Bio supplies Cat# CYRN03 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
RNApure FAQ
Does this kit use phenol-chloroform?
Partially: lysis uses Buffer RL, a TRIzol-style monophasic reagent, and the protocol adds 0.2 ml chloroform (or 50 µl 4-bromoanisole) per 1 ml Buffer RL before phase separation. The RNA is then purified on silica columns — no alcohol precipitation. For a fully phenol/chloroform-free workflow, see our Plus RNA Mini Kit (CYRN28).
Can vortexing increase DNA contamination?
Yes — the manual warns that vortexing may increase DNA contamination in the RNA sample. If your downstream application is DNA-sensitive, avoid vortexing and use the on-column DNase Digestion Kit (CYRN3401) or a post-purification DNase step.
What about storage?
Buffer RL must be stored at 4°C (the only refrigerated component); other components are RT. All components are stable 9 months. Keep purified RNA on ice for short-term use or at −80°C long term.
Cat# CYRN03 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.
International ordering — CYRN03
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Storage: Buffer RL at 4°C; other reagents at RT; stable for 9 months; shipped ambient (room temperature); no ice packs. Shipping conditions are confirmed per item.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYRN03 — tell us the quantity and destination city.
Alternative-product and purchasing questions
Is CYRN03 an alternative for a TRIzol Plus RNA purification workflow?
CYRN03 RNApure combines a TRIzol-style lysis and phase-separation workflow with RNA spin-column purification. It is an option to evaluate for research RNA isolation when both steps fit your sample. It is a kit workflow, not simply a bottle-for-bottle reagent substitution.
Is CYRN03 a phenol-free replacement for an RNeasy-style kit?
No. CYRN03 uses organic extraction and requires the relevant handling precautions and materials described in its manual. For an appropriate phenol/chloroform-free animal-cell or tissue workflow with gDNA elimination, review CYRN28 instead.
How do I request a quotation for CYRN03?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Include the sample type and downstream application.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.