Combines TRIzol®-grade lysis (Buffer RL) with silica spin-column purification to isolate ultrapure total RNA from animal and plant cells and tissues in under an hour. Superior lysis power with a convenient, time-saving protocol.
Key Features
- TRIzol®-based lysis + spin-column purification
- Superior lysis capability for cells and tissues
- Ultrapure total RNA in under one hour
- No phenol/chloroform phase separation needed
Specifications
| Pack size | 50 Preps (CYRN0302) |
| Components | Buffer RL (TRIzol® Reagent), Wash Buffer RE, Wash Buffer RW, RNase-free H2O, RNA Bind Columns |
| Storage | Buffer RL at 4°C; other reagents at RT; stable for 9 months |
| Shipping | Ambient (room temperature); no ice packs |
| Note | Add indicated ethanol to Wash Buffer RW before first use |
Typical Applications
- Total RNA from animal or plant tissue, or cultured cells, using Buffer RL (TRIzol) plus a spin column.
- Aqueous-phase RNA after chloroform separation.
- Optional on-column DNase if the downstream step needs DNA-free RNA.
Input ranges at a glance
| Tissues | 50–100 mg per 1 ml Buffer RL; sample volume must not exceed 10% of the Buffer RL volume |
| Adherent cells | Lyse directly in the dish: 1 ml Buffer RL per 10 cm² (do not wash cells before adding Buffer RL) |
| Suspension cells | 5–10 × 10⁶ animal/plant/yeast cells or 1 × 10⁷ bacterial cells per 1 ml Buffer RL |
| Aqueous phase | About 500–600 µl after centrifugation; transfer ~480 µl |
| Ethanol | 0.5 volume (240 µl) — 33% final concentration |
| Elution | 30–50 µl RNase-free water; 70–90°C pre-warming raises yield |
| Storage / pack sizes | Buffer RL (TRIzol) at 4°C, other components RT; stable 9 months; CYRN0302 (50 preps) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped at ambient temperature (no ice packs).
- Buffer RL at 4°C; other reagents at room temperature. Stable 9 months at the indicated temperatures.
- Sample volume should not exceed 10% of Buffer RL. Vortexing can increase DNA contamination of RNA.
- Purified RNA: on ice if used within hours; −80°C for long-term storage. On-column DNase is a separate kit (not included).
Who sets these specifications
Changyu Bio manufactures Cat# CYRN03 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
RNApure FAQ
Does this kit use phenol-chloroform?
Partially: lysis uses Buffer RL, a TRIzol-style monophasic reagent, and the protocol adds 0.2 ml chloroform (or 50 µl 4-bromoanisole) per 1 ml Buffer RL before phase separation. The RNA is then purified on silica columns — no alcohol precipitation. For a fully phenol/chloroform-free workflow, see our Plus RNA Mini Kit (CYRN28).
Can vortexing increase DNA contamination?
Yes — the manual warns that vortexing may increase DNA contamination in the RNA sample. If your downstream application is DNA-sensitive, avoid vortexing and use the on-column DNase Digestion Kit (CYRN3401) or a post-purification DNase step.
What about storage?
Buffer RL must be stored at 4°C (the only refrigerated component); other components are RT. All components are stable 9 months. Keep purified RNA on ice for short-term use or at −80°C long term.
Cat# CYRN03 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.