Purifies genomic-DNA-free total RNA from animal cells or tissues using unique genomic DNA Elimination Columns — no DNase digestion step required. Phenol/chloroform-free, highly reproducible yields in minutes.
Key Features
- Unique gDNA Elimination Columns avoid the need for DNase
- Efficient removal of genomic DNA
- Highly reproducible RNA yields in minutes
- High-performance RNA for sensitive downstream applications
Specifications
| Pack size | 50 Preps (CYRN28) |
| Components | Buffer RLT Plus, Buffer RW1, Wash Buffer RW, 70% Ethanol, RNase-free H2O, gDNA Elimination Columns, RNA Binding Columns |
| Storage | RT; stable for 9 months at indicated temperatures |
| Shipping | Ambient (room temperature); no ice packs |
| Note | Add indicated ethanol before first use |
Typical Applications
- Total RNA from animal cells or tissues using a gDNA-elimination column (no DNase step in the standard protocol).
- Manual tissue examples include liver and brain; cultured cells as listed in the protocol.
- RNA for RT-qPCR where residual genomic DNA is a problem.
Input ranges at a glance
| Cultured cells | <5×10⁶ cells in 350 μl lysis buffer; 5×10⁶–1×10⁷ cells in 600 μl |
| Animal tissue | <20 mg in 350 μl; 20–30 mg in 600 μl |
| Manual tissue examples | Mouse liver and brain |
| gDNA removal | gDNA elimination column: gDNA binds to the membrane, RNA stays in the flow-through — no DNase step |
| Protocol | Phenol/chloroform-free; lysis buffer immediately inactivates RNase and DNase |
| Storage | All components at room temperature; stable 9 months at the indicated temperatures |
| Pack size | CYRN2802, 50 preps |
All values from the CYRN28 manual (PDF).
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped at ambient temperature (no ice packs).
- Store at room temperature (manual: 9 months). Add the indicated ethanol before first use.
- gDNA is removed on the elimination column — a separate DNase digest is usually unnecessary, but follow the manual if your assay is DNA-sensitive.
Who sets these specifications
Changyu Bio manufactures Cat# CYRN28 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
CYRN28 FAQ
- Does CYRN28 work on fibrous or dense tissue? The manual documents animal tissues (examples: mouse liver and brain) and cultured cells. It does not claim validation on fibrous or collagen-rich tissue — validate on your tissue type before committing samples.
- Is a DNase digestion step needed? No. The gDNA elimination column binds genomic DNA while RNA stays in the flow-through, so the standard protocol has no DNase step. For DNA-sensitive assays, follow the manual.
- Is the protocol phenol/chloroform-free? Yes — the whole process is phenol/chloroform-free, and the lysis buffer immediately inactivates RNase and DNase.
- How can I improve RNA yield? Preheat the RNase-free water to 70–90°C for the elution step (manual). For elution volumes above 700 μl, load in two passes.
Cat# CYRN28 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery worldwide.