Designed for rapid purification of total RNA from plants — including complex, polysaccharide- and polyphenol-rich species. Genomic DNA Elimination Columns remove gDNA without DNase; the whole process is phenol/chloroform-free.
Key Features
- Handles complex plant samples with high secondary-metabolite content
- gDNA Elimination Columns — no DNase digestion step
- Phenol/chloroform-free workflow
- Lysis buffer immediately inactivates RNase and DNase
Specifications
| Pack size | 50 Preps (CYRN53) |
| Components | Lysis Buffer CLB, Lysis Buffer RLT Plus, Buffer RW1, Wash Buffer RW, RNase-free H2O, gDNA Elimination Columns, RNA Binding Columns |
| Storage | RT (15–25°C); do not refrigerate — low temperature causes precipitation |
| Shipping | Ambient (room temperature); no ice packs |
| Note | Keep container tightly sealed after use |
Typical Applications
- Total RNA from polysaccharide- or polyphenol-rich plants (manual examples: Dendrobium, Salvia, ginseng).
- Typical OD260/280 about 2.0–2.2 without a separate DNase digest.
- Ship and store 15–25°C — do not refrigerate the kit.
Input ranges at a glance
| Sample | 100–200 mg fresh or frozen plant tissue in 1 ml CLB working lysis buffer (with β-mercaptoethanol) |
| Grinding | Direct grinding (no liquid nitrogen) or liquid-nitrogen grinding for difficult, degradable samples |
| Purity | Typical OD260/OD280 2.0–2.2 with no DNA contamination |
| Time | Single extraction completed in about 30 minutes |
| gDNA removal | Built-in genomic DNA elimination column — DNase digestion generally not needed |
| Elution | 30–50 µl RNase-free water; 70–90°C pre-warming raises yield |
| Storage / pack sizes | RT only (15–25°C transport and storage — cold storage causes precipitation); stable 9 months; CYRN53 (50 preps) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped at ambient temperature (no ice packs).
- Shipped and stored at ambient temperature (15–25°C). Do not refrigerate or freeze the buffers — 4°C or −20°C causes precipitation and loss of performance.
- Keep bottles tightly closed. β-mercaptoethanol in Buffer CLB is required as in the manual.
- Add the indicated ethanol to Wash Buffer RW before first use.
Who sets these specifications
Changyu Bio supplies Cat# CYRN53 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
Complex Plant RNA FAQ
Which difficult plants has this kit been validated on?
The manual lists complex medicinal herbs — Dendrobium, Salvia, Saussurea and ginseng; starch-rich seeds — rice, wheat and corn; and fruits — grape, blueberry, strawberry and watermelon.
Is DNase digestion needed?
Generally no — the gDNA elimination column removes most genomic DNA, and the manual reports typical OD260/OD280 ratios of 2.0–2.2 with no DNA contamination. For trace-DNA-sensitive assays (e.g., low-abundance TaqMan RT-PCR), use RNase-Free DNase I on-column, or design intron-spanning primers.
Why must this kit stay at room temperature?
Cold storage (4°C or −20°C) causes precipitation that affects reagent performance. Store and transport the kit at 15–25°C and close bottles promptly after use. If CLB precipitates anyway, redissolve at 65°C.
What if my plant tissue is especially difficult?
Raise β-mercaptoethanol to 10–20% final in the CLB working solution if needed, and add PVP40 to 2% final for very complex plants (manual).
Cat# CYRN53 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.
International ordering — CYRN53
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Storage: RT (15–25°C); do not refrigerate — low temperature causes precipitation; shipped ambient (room temperature); no ice packs. Shipping conditions are confirmed per item.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYRN53 — tell us the quantity and destination city.
Alternative-product and purchasing questions
Can CYRN53 be considered when looking for an alternative to a RNeasy Plant workflow?
CYRN53 is an option for reviewing total RNA extraction from difficult plant material, including polysaccharide- or polyphenol-rich samples within its manual’s scope. Match the actual plant sample and required workflow rather than assuming all plant RNA kits are equivalent.
Can I use a RNeasy Plant protocol unchanged with CYRN53?
Follow the CYRN53 lysis, gDNA-elimination and RNA-binding instructions. Provide the plant species, tissue, preservation conditions and downstream assay when asking about suitability. Evaluate the kit before transferring an established study.
How do I request a quotation for CYRN53?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Include the sample type and downstream application.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.