Spin-column DNA purification kit for cells and tissues. Samples are lysed with Proteinase K under chaotropic conditions that immediately inactivate nucleases; DNA binds selectively to the silica membrane and is purified through rapid wash-and-spin steps. A dedicated Inhibitor Removal Buffer handles difficult samples.
Key Features
- No toxic phenol/chloroform, no organic extraction or ethanol precipitation
- Multipurpose — one kit for a variety of sample materials
- Inhibitor Removal Buffer (Buffer IR) for demanding samples
- Fast, easy protocol with high-quality DNA yield
Specifications
| Pack sizes | 50 Preps (CYDN0701) / 100 Preps (CYDN0702) |
| Components | Buffer TL, Buffer CB, Buffer IR, Buffer WB, Elution Buffer, Proteinase K (20 mg/ml), DNA Bind Columns |
| Storage | Buffers at RT; Proteinase K at -20°C |
| Shipping | Ambient (room temperature); no ice packs |
| Note | Add indicated ethanol to Buffer WB before first use |
Typical Applications
- Genomic DNA from cultured cells after Proteinase K lysis.
- Animal tissue: about 20–40 mg (spleen up to 10 mg) per the manual.
- Optional RNase A when RNA-free genomic DNA is required for PCR or similar research use.
Input ranges at a glance
| Blood (non-nucleated RBC) | 200 µl anticoagulated whole blood; top up with PBS if less than 200 µl |
| Blood (nucleated RBC — bird/fish/frog) | 10–20 µl, top up to 200 µl with PBS |
| Cultured cells | Up to 5 × 10⁶ cells, resuspended in 200 µl PBS |
| Animal tissue | 20–40 mg (spleen up to 10 mg); digest at 56°C for 1–3 hours |
| Mouse tail | 0.2–0.5 cm (25–50 mg); digest 3 hours or overnight |
| Heparin tolerance | Buffer IR removes inhibitors — supports heparinized samples up to 100 U/ml heparin |
| Elution | 100 µl standard, minimum 30 µl; pre-warming elution buffer to 70–90°C raises yield |
| Storage / pack sizes | RT components, Proteinase K −20°C; stable 9 months; CYDN0701 (50 preps) · CYDN0702 (100 preps) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped at ambient temperature (no ice packs).
- Buffers at room temperature; Proteinase K at −20°C after reconstitution. Avoid repeated freeze–thaw of Proteinase K. Kit stable 9 months at the indicated temperatures.
- Add the indicated ethanol to Buffer WB before first use. Residual ethanol on the column membrane interferes with downstream reactions — dry-spin as in the manual.
- Optional RNase A is only if RNA-free genomic DNA is required.
Who sets these specifications
Changyu Bio supplies Cat# CYDN07 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
Plus DNA Mini Kit FAQ
Can I use heparinized blood samples?
Yes. The kit includes an Inhibitor Removal Buffer (Buffer IR) that supports samples containing up to 100 U/ml heparin — a key difference from standard blood DNA kits.
How can I improve DNA yield?
Pre-heat the Elution Buffer to 70–90°C before elution, and elute twice by passing the eluate back through the column — the manual reports about 10–15% higher concentration with a second elution.
What digestion times does the manual give?
Animal tissue: 56°C for 1–3 hours; mouse tail: 3 hours or overnight. All components are stable for 9 months at the indicated temperatures; Proteinase K is stored at −20°C.
Cat# CYDN07 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.
Alternative-product and purchasing questions
Is CYDN07 an alternative to QIAamp DNA Mini 51304 for cells or tissues?
CYDN07 Plus DNA Mini is an option for research DNA preparation from cells or tissues using a proteinase K and silica-column workflow. Confirm that your particular sample is covered by the CYDN07 manual; this is not a claim that it replaces every QIAamp sample protocol.
Can I keep the QIAamp protocol when switching to CYDN07?
Follow the CYDN07 instructions for lysis, binding, washing and elution. Do not mix buffer identities or carry over a different kit’s protocol without validation. Provide your sample type and downstream application when requesting a recommendation.
How do I request a quotation for CYDN07?
Include the current kit name or catalog number, requested quantity, delivery country and city, and the application details above. Ask us to confirm the available pack size, price and delivery conditions in writing. Include the sample type and downstream application.
For laboratory research use only. Other brands identify the workflow being considered; no affiliation or endorsement is implied.