Purifies DNA (100 bp – 10 kb) from standard or low-melt agarose gels in TAE/TBE buffer. Up to 400 mg agarose per spin column; colored Buffer DD makes dissolving, binding and washing steps easy to monitor visually.
Key Features
- Purifies 100 bp – 10 kb DNA from agarose gels
- Handles up to 400 mg agarose per column
- Colored gel-dissolving buffer for visual monitoring
- Quick recovery of ultra-pure, ready-to-use DNA
Specifications
| Pack sizes | 50 Preps (CYDR0101) / 100 Preps (CYDR0102) |
| Yield | Up to 10 µg DNA per preparation |
| Components | Buffer DD, Buffer WB, Elution Buffer, DNA Binding Columns |
| Storage | RT; stable for 12 months at indicated temperatures |
| Shipping | Ambient (room temperature); no ice packs |
| Note | Add indicated ethanol to Buffer WB before first use |
Typical Applications
- Recover 100 bp–10 kb DNA from TAE or TBE agarose slices (up to 400 mg gel; up to 10 μg DNA).
- Cleanup of bands before cloning or sequencing in research workflows.
- Add isopropanol after dissolving the gel for fragments <500 bp or >4 kb (manual).
Input ranges at a glance
| Gel slice | Up to 400 mg agarose per column (TAE or TBE, standard or low-melting); split larger slices |
| Dissolving buffer | 3 volumes Buffer DD per gel volume (100 mg ≈ 100 µl); 6 volumes for >2% agarose gels |
| PCR cleanup mode | 5 volumes Buffer DD per 1 volume PCR sample (same column workflow) |
| Fragment range / capacity | 100 bp to 10 kb; up to 10 µg PCR product per column |
| Isopropanol step | 150 µl per 100 mg gel — improves yield for fragments <500 bp and >4 kb |
| Elution | 50 µl standard, minimum 30 µl; 70–90°C pre-warmed elution buffer raises yield |
| Storage / pack sizes | All components RT, stable 12 months; CYDR0101 (50 preps) · CYDR0102 (100 preps) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped at ambient temperature (no ice packs).
- Intended for DNA of about 100 bp to 10 kb from standard or low-melt agarose in TAE or TBE. Up to 400 mg agarose per spin column.
- Store at room temperature (manual: 12 months). Add ethanol to Buffer WB before first use.
- Residual ethanol on the membrane interferes with ligation and other downstream steps — complete the dry spin.
Who sets these specifications
Changyu Bio manufactures Cat# CYDR01 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
Gel Extraction Kit FAQ
When does the isopropanol step actually help?
The manual states isopropanol improves yield for fragments smaller than 500 bp and larger than 4 kb; for 500 bp–4 kb fragments it has no effect on yield. Add it after the gel has completely dissolved and do not centrifuge at that stage.
Can I also purify PCR products with this kit?
Yes — the manual includes a PCR cleanup protocol: mix 5 volumes of Buffer DD with 1 volume of PCR sample and follow the same column steps. For routine PCR cleanup you may also want the dedicated PCR Purification Kit (CYDR02).
What agarose concentrations are supported?
TAE or TBE gels, standard or low-melting point. The colored Buffer DD helps you visually confirm dissolving, binding and washing. For >2% gels use 6 volumes of Buffer DD and extend the 56°C incubation until the gel is completely dissolved.
Cat# CYDR01 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.