Removes primers, nucleotides and salts from PCR products (100 bp – 10 kb) by selective silica-membrane binding in the presence of guanidine thiocyanate. No organic extraction or precipitation — up to 95% recovery of ready-to-use DNA.
Key Features
- Purifies up to 10 µg PCR products, 100 bp – 10 kb
- Up to 95% recovery of ready-to-use DNA
- Removes primers, nucleotides and enzymes in three easy steps
- No organic solvents or ethanol precipitation
Specifications
| Pack sizes | 50 Preps (CYDR0201) / 100 Preps (CYDR0202) |
| Yield | Up to 10 µg DNA; up to 95% recovery |
| Components | Buffer PB, Buffer WB, Elution Buffer, DNA Binding Columns |
| Storage | RT; stable for 12 months at indicated temperatures |
| Shipping | Ambient (room temperature); no ice packs |
| Note | Add indicated ethanol to Buffer WB before first use |
Typical Applications
- Clean PCR products 100 bp–10 kb (up to 10 μg) without cutting a gel.
- Remove primers and dNTPs on a silica column after PCR.
- Mineral oil or kerosene need not be removed first.
Input ranges at a glance
| PCR sample | 5 volumes Buffer PB per 1 volume PCR sample (e.g., 500 µl per 100 µl) |
| Mineral oil | Not necessary to remove mineral oil or kerosene before loading |
| Fragment range / capacity | 100 bp to 10 kb; up to 10 µg PCR product per column |
| Recovery | Up to 95% of ready-to-use DNA |
| Workflow | Three easy steps: bind, wash, elute |
| Elution | 50 µl standard, minimum 30 µl; 70–90°C pre-warmed elution buffer raises yield |
| Storage / pack sizes | All components RT, stable 12 months; CYDR0201 (50 preps) · CYDR0202 (100 preps) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped at ambient temperature (no ice packs).
- Store at room temperature (manual: 12 months). Add ethanol to Buffer WB before first use.
- Residual ethanol on the membrane interferes with downstream reactions — complete the dry spin.
Who sets these specifications
Changyu Bio manufactures Cat# CYDR02 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
PCR Purification Kit FAQ
What recovery can I expect?
Up to 95% recovery of ready-to-use DNA for fragments from 100 bp to 10 kb (manual). Elute with buffer pre-warmed to 70–90°C and run the eluate through the column a second time for about 10–15% higher concentration.
Do I need to remove the mineral oil overlay from my PCR first?
No — the manual states it is not necessary to remove mineral oil or kerosene before loading the sample.
What is the difference between this kit and the Gel Extraction Kit?
This kit purifies PCR products directly from the reaction. If you need to cut a specific band from an agarose gel first, use the Gel Extraction Kit (CYDR01) — same column chemistry, same 100 bp–10 kb range.
Cat# CYDR02 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.