High-Specificity Taq DNA Polymerase for ARMS and SNP Detection
An engineered Taq DNA polymerase that recognises a single-base mismatch at the primer 3′ end, so ARMS assays need only one divergent base – no extra mismatches or loop primers.
For research use only. Not for use in diagnostic procedures. Price on request – written quotation by email.
Overview
Developed by semi-rational directed evolution of wild-type Taq, this polymerase shows markedly higher primer specificity, reducing NTC and non-specific amplification. It suits SNP analysis, genotyping, tumour mutation research, pathogen identification and gene-editing efficiency analysis.
Specifications
Manufacturer specifications. Final configuration is confirmed in the quotation.
| Item No. | Size | Version |
|---|---|---|
| CY-PCR-06-S1 / H1 | 50 µL (250 U), 5 U/µL | Standard / Hot-start |
| CY-PCR-06-S2 / H2 | 200 µL (1,000 U), 5 U/µL | Standard / Hot-start |
| CY-PCR-06-S3 / H3 | 2 mL (10,000 U), 5 U/µL | Standard / Hot-start |
| Formats | Probe method, electrophoresis, dye method |
| Components | Enzyme (with glycerol), 5× buffer, user manual; buffer also sold separately |
| Enzyme : buffer | 1 : 10 |
| Storage / shelf life | −25 to −15 °C / 2 years |
| Customisation | Buffer without ions or dNTP on request |
Applications
Typical research uses.
Frequently Asked Questions
Answers for buyers and lab managers.
Why is it useful for ARMS PCR?
Because it discriminates a single 3′ mismatch, primers need only the one diagnostic base – design is much simpler than first- or second-generation ARMS.
Which platforms is it compatible with?
Digital PCR, qPCR and conventional PCR.
Is there a hot-start version?
Yes, every size is available as hot-start or standard enzyme.
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Send the item numbers, quantities and destination. We reply by email with a written quotation; lead time, power supply (voltage/plug), documentation and shipping are confirmed per order.