CYPC85 is a one-step qRT-PCR kit for probe-based real-time quantitative detection from RNA. Reverse transcription and quantification run consecutively in one tube.
Catalog no. CYPC85 · For research use only
Key features
- Probe method; single-tube reverse transcription and real-time detection
- Closed-tube format helps prevent contamination and reduces pipetting error
- Efficient reverse transcriptase, hot-start polymerase and optimized buffer, with good results on GC-rich or structured RNA
- Suited to low-abundance targets such as RNA viruses
- Add template, primers, probe, ROX Reference Dye (instrument-dependent) and water to 1x; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC8501 | TRUEscript One Step qRT-PCR Probe Kit | 100 reactions x 20 μL |
| CYPC8502 | TRUEscript One Step qRT-PCR Probe Kit | 200 reactions x 20 μL |
Specifications
| Format | One-step qRT-PCR kit, probe method |
|---|---|
| Reaction size | 20 μL |
| ROX | ROX Reference Dye, used depending on instrument |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- RNA virus detection
- Probe-based one-step qRT-PCR
- Low-abundance target detection
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
TRUEscript One Step qRT-PCR Probe Kit FAQ
What is the ROX Reference Dye for?
It corrects well-to-well fluorescence differences; use depends on the real-time PCR instrument.
What do I add to the reaction?
Template, primers, probe, ROX Reference Dye if needed, and water, to a 1x working concentration.
What is the storage condition?
-20 °C, 12-month shelf life.
How do I order CYPC85?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.