RayBiotech offers three m6A kits: a quantitative competitive ELISA (EIA-m6A) for plasma, serum and urine; a semi-quantitative dot blot (DB-m6A) that also accepts extracted RNA; and a MeRIP-qPCR kit (QMERIP-M6A) for m6A enrichment on specific genes. Pick by sample type and by whether you need a global level or a gene-level answer. Request a quote for the m6A ELISA kit.
Compare the three m6A kits
| EIA-m6A (ELISA) | DB-m6A (Dot Blot) | QMERIP-M6A (MeRIP-qPCR) | |
|---|---|---|---|
| Question answered | m6A level in biofluid samples | Global m6A comparison between samples | m6A enrichment on genes of interest |
| Output | Quantitative | Semi-quantitative | Quantitative |
| Listed sample types | Plasma, serum, urine | Plasma, serum, extracted RNA | Extracted RNA |
| Species | Human, mouse, rat | Human, mouse, rat | Human, mouse, rat |
| Sizes | 1, 2 or 5 plates | 1 kit (at least 48 samples) | 48 or 96 tests |
| Readout / instrument | Absorbance at 450 nm; microplate reader | Chemiluminescence; UV cross-linker and imaging system | qPCR; fluorescence PCR instrument and magnetic separator |
| Kit storage | -20 to -80 °C | -20 to -80 °C | -20 or -80 °C |
| Quote | EIA-m6A | DB-m6A | QMERIP-M6A |
Specifications as listed by RayBiotech for each catalog number; follow the manual shipped with the kit.
m6A ELISA kit (EIA-m6A)
This competitive assay uses a 96-well strip plate and a standard curve to report m6A concentration. RayBiotech lists a sensitivity of 0.435 ng/ml and a range of 0.435 to 10,000 ng/ml. Antibody specificity was checked by RayBiotech against m1A, m7G and m5C. Each plate covers up to 96 wells, including standards.
m6A dot blot kit (DB-m6A)
Samples are spotted on nitrocellulose, UV cross-linked and probed with an anti-m6A antibody. Methylene blue staining of a replicate line serves as the internal control. It suits fast comparison across many samples when you do not need an absolute concentration.
m6A MeRIP-qPCR kit (QMERIP-M6A)
RNA is fragmented, enriched with magnetic-bead m6A antibody and read by qPCR. The kit includes a non-immune IgG control and control primers (SMAD7 positive, ACTB negative). RayBiotech lists about 5 hours in total, with under 1 hour hands-on. The enriched RNA can also be used for transcriptome-wide profiling.
Not sure which method fits?
Read our guide to ELISA, dot blot, MeRIP-qPCR and LC-MS/MS for m6A, or send your sample type, species and sample count with your quote request.
Common questions
Can the m6A ELISA kit measure purified RNA?
RayBiotech lists plasma, serum and urine as compatible samples for EIA-m6A. Extracted RNA is listed for the dot blot kit (DB-m6A) and the MeRIP-qPCR kit (QMERIP-M6A), so choose one of those for purified RNA.
Which species are the m6A kits validated for?
RayBiotech lists human, mouse and rat for EIA-m6A, DB-m6A and QMERIP-M6A.
What is the sensitivity and range of the m6A ELISA kit?
RayBiotech lists a sensitivity of 0.435 ng/ml and a detection range of 0.435 to 10,000 ng/ml for EIA-m6A.
Are these kits for diagnostic use?
No. They are research use only.
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