Freund’s Adjuvant

$249.00

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Description

Product Manual

Cat# Size Product Description Price ($)
CY0210041 1ml 5 weeks mouse monoclonal/polyclonal antibody quick adjuvant 249
CY0210042 1ml 3 weeks mouse monoclonal/polyclonal antibody quick adjuvant 249
CY0210043 1ml 2 weeks mouse polyclonal antibody quick adjuvant 249
CY0210045 1ml 8 weeks rabbit polyclonal antibody quick adjuvant 249

 

Transportation and Storage

Transportation at Room Temperature

The adjuvant is stable for up to 18 months when stored at 2-8ºC.

 

Our QuickAntibody Adjuvant, in other word, this original Freund’s Adjuvant is a proprietary novel adjuvant designed specifically for the preparation of monoclonal as well as polyclonal antibodies in mice. Compared with the conventional Freund’s adjuvant, this original Freund’s Adjuvant has the unique features

 

Parameter Comparison Changyu Quickantibody Adjuvant Freund’S Adjuvant The Advantage Of Changyu Quickantibody Adjuvant
Emulsification No Yes Save at least 30 mins-2 hours tedious emulsification
Immune Method Easily intra-muscular injection Tedious foot-pad or intra-spleen immunization Much easier immunization
Immunization Schedule 2-5 weeks 8-10 weeks Save at least 3-5 weeks time
Immunization Times 2 times 3-4 times Less immunization times
Destroy of Natural Antigen Conformation No Yes Facilitating the generation of monoclonal antibodies against natural conformational epitopes
Toxicity No Yes Safe to animal
Antigen Dosage 1-20ug/dose 50-100ug/dose Save your precious antigen
Immunogenicity No Yes Much higher positive rate of expected specific monoclonal antibodies

 

User Guidelines

  1. Original Freund’s Adjuvant can achieve antigen-sparing effect through fewer immunizations (only two injections) and less antigen dosage per injection. Recommended antigen dosage is: (1) 5~50μg (5-50μg for mouse, 20-50μg for rabbit) per injection for low immunogenic antigens such as recombinant subunit proteins or peptide-carrier conjugates; (2) 1~10μg (1-5μg for mouse, 5-10μg for rabbit) per injection for high immunogenic antigens such as inactivated viruses or recombinant virus-like particles.
  2. Original Freund’s Adjuvant can induce a quick immune response, generating high titers as well as high affinity of antibodies. Whatever used for monoclonal as well as polyclonal antibody preparation, it requires only two immunizations within 3 weeks, and high titers of antibodies with ELISA titers (Cutoff=0.1000) up to 1:10000~1:10000000 could be elicited in the fifth week.
  3. In contrast to Freund’s adjuvant, Original Freund’s Adjuvant doesn’t destroy natural antigen conformation, which facilitates the generation of monoclonal antibodies against conformational epitopes.
  4. In contrast to complete Freund’s adjuvant, Original Freund’s Adjuvant doesn’t have protein antigens, which eliminates the epitope competition from adjuvant-derived proteins.
  5. In contrast to the tedious emulsification required by Freund’s adjuvant, Original Freund’s Adjuvant is provided as a ready-to-use solution that can be easily mixed with any antigen before animal immunization.
  6. In contrast to the tedious foot-pad or intra-spleen immunization usually used in monoclonal antibody protocols, Original Freund’s Adjuvant can be conveniently used through intra-muscular injection.
  7. Original Freund’s Adjuvant is most useful in preparing murine polyclonal antibodies. In contrast to conventional rabbit polyclonal antibody preparation, which is usually provided by companies as customer-service, with Original Freund’s Adjuvant any laboratory can easily generate 1ml of high quality murine polyclonal antibodies within 5 weeks by simply immunizing 5 mice. (Note: 1ml of high quality murine polyclonal antibodies should fulfill most experimental requirements.)
  8. Original Freund’s Adjuvant is shipped at ambient temperature and stored at 2-8ºC (Do not freeze!).

 

Need help? Contact us immediately: info@changyubio.com.

 

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Shipping Method :  FedEx

Shipping/Freight  Charges:- Will be beared by Buyer

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