CYRN65 is a chloroform-free upgrade of the TRIzol-type workflow for isolating total RNA and small RNA from animal tissue, plant material, cultured cells and bacteria. No chloroform is needed for phase separation and the process runs at room temperature.
Catalog no. CYRN65 · For research use only
Key features
- No chloroform phase separation; simpler operation at room temperature
- Isolated RNA has essentially no DNA residue
- RNA usable for cDNA cloning, qRT-PCR, mRNA purification, in vitro translation, Northern blotting and high-throughput sequencing
- Works well on common animal tissue and cells (liver, kidney, brain, cultured cells) and simple plant tissue (rice, maize, Arabidopsis, tobacco, wheat)
- Not suitable for polysaccharide/polyphenol-rich plants such as cotton or certain hard-to-lyse bacteria
- Research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYRN6501 | Chloroform-Free Total RNA Isolation Reagent | 50 mL |
| CYRN6502 | Chloroform-Free Total RNA Isolation Reagent | 100 mL |
Specifications
| Format | Liquid reagent, chloroform-free |
|---|---|
| Sample | Animal tissue, plant material, cultured cells, bacteria |
| RNA | Total RNA and small RNA |
| Temperature | Room temperature |
| Use | Research use only |
Applications
- cDNA cloning
- qRT-PCR
- mRNA purification
- Northern blotting
- High-throughput sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Chloroform-Free Total RNA Isolation Reagent FAQ
Is chloroform used?
No. Phase separation does not require chloroform.
Which samples is it not suited to?
Polysaccharide/polyphenol-rich plants such as cotton and some hard-to-lyse bacteria.
Does it need low temperature?
No, the whole procedure can run at room temperature.
How do I order CYRN65?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.