CYRN54 is a spin-column total RNA kit for bone tissue and cells, including mineralized bone. Its lysis solution contains no phenol, chloroform or beta-mercaptoethanol and includes components that remove bone proteoglycans.
Catalog no. CYRN54 · For research use only
Key features
- Bone is hard, low in cell density and rich in proteoglycan matrix, which is difficult for TRIzol-type extraction
- Combined DNA removal/RNA binding column; RNA generally needs no DNase digestion
- Single sample usually within 30 minutes
- Typical OD260/OD280 of 1.9-2.2 with essentially no DNA residue
- RNA usable for RT-PCR, RT-qPCR, Northern blot, next-generation sequencing and array analysis; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYRN5401 | EASYspin Plus Bone Tissue RNA Kit | 50 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Sample | Bone tissue (including mineralized bone) and cells |
| Time | About 30 minutes |
| Typical OD260/OD280 | 1.9-2.2 |
| Use | Research use only |
Applications
- RT-PCR and RT-qPCR
- RNA-seq library preparation
- Microarray analysis
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
EASYspin Plus Bone Tissue RNA Kit FAQ
Does it work on mineralized bone?
Yes, it is described as suited to various bone tissues including mineralized bone.
Are toxic reagents used?
No phenol, chloroform or beta-mercaptoethanol.
Is DNase digestion needed?
Generally not.
How do I order CYRN54?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.