Fastest, easiest way to purify viral RNA for downstream amplification — from plasma (non-heparin anticoagulants), serum and other cell-free body fluids. Consistent, reproducible results with complete removal of contaminants and inhibitors.
Key Features
- Rapid isolation of high-quality, ready-to-use RNA
- No organic extraction or alcohol precipitation
- Consistent, reproducible results
- Complete removal of contaminants and inhibitors
Specifications
| Pack size | 50 Preps (CYRN2701) |
| Sample types | Plasma (non-heparin anticoagulant), serum, cell-free body fluids |
| Components | Buffer RLB, Wash Buffer RE, Wash Buffer RW, RNase-free H2O, RNA Bind Columns |
| Storage | RT; stable for 9 months at indicated temperatures |
| Shipping | Ambient (room temperature); no ice packs |
| Note | Avoid repeated freeze-thaw of plasma samples |
Typical Applications
- Viral RNA from 200 μl plasma, serum, urine, culture supernatant, or other cell-free fluid (research samples).
- Templates for research RT-PCR / RT-qPCR amplification workflows.
- Research Use Only — not a diagnostic specimen collection or IVD kit.
Input ranges at a glance
| Sample | 200 µl plasma, serum, urine, cell culture supernatant or other cell-free body fluid + 400 µl Buffer RLB |
| Low-volume samples | Top up to 200 µl with PBS |
| Plasma anticoagulant | Any anticoagulant except heparin (heparinized plasma is not supported) |
| Lysis | 10 minutes at room temperature (15–25°C); infectious agents and RNases inactivated in Buffer RLB |
| Ethanol | 450 µl 96–100% ethanol, pulse-vortex 15 s |
| Elution | 30–50 µl RNase-free water; 70–90°C pre-warming raises yield |
| Storage / pack sizes | All components RT, stable 9 months; CYRN2701 (50 preps) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use. Not an IVD kit.
- Shipped at ambient temperature (no ice packs).
- Plasma should not be heparin-treated. Store kit at room temperature (manual: 9 months).
- Purified RNA: on ice if used within hours; −80°C for long-term storage. Avoid repeated freeze–thaw of plasma samples.
Who sets these specifications
Changyu Bio manufactures Cat# CYRN27 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
Viral RNA Mini Kit FAQ
Can I use heparinized plasma?
No — the manual specifies plasma treated with anticoagulants other than heparin. Heparinized samples are not supported by this kit.
Can I freeze and thaw my samples?
Use fresh or frozen samples, but do not thaw frozen samples more than once — repeated freeze-thaw lowers viral titer and can produce cryoprecipitates that clog the membrane on vacuum protocols.
Does longer lysis improve yield?
No. Viral particles are completely lysed after 10 minutes, and longer incubation does not change RNA yield or quality (manual).
Cat# CYRN27 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.