CYPP25 is a BCIP/NBT chromogenic substrate kit for alkaline phosphatase (AP). AP fixed by immunoreaction catalyzes BCIP/NBT to form a blue-purple compound that precipitates in situ at positive protein bands on a blot membrane. The kit contains AP reaction buffer, NBT stock and BCIP stock.
Catalog no. CYPP25 · For research use only
Key features
- BCIP/NBT chromogenic substrate for alkaline phosphatase
- Blue-purple precipitate forms in situ at positive bands on blot membranes
- Includes 100 mL AP reaction buffer, 1 mL NBT stock, 1 mL BCIP stock
- NBT and BCIP stocks stored at -20 °C away from light
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPP2501 | BCIP/NBT Alkaline Phosphatase Color Development Kit | 100 mL |
Specifications
| Format | Chromogenic substrate kit |
|---|---|
| Enzyme | Alkaline phosphatase (AP) |
| Product colour | Blue-purple precipitate |
| AP reaction buffer | 100 mL, room temperature or 4 °C |
| NBT stock | 1 mL, -20 °C, protect from light |
| BCIP stock | 1 mL, -20 °C, protect from light |
| Use | Research use only |
Applications
- Chromogenic detection of AP-labelled antibodies on Western blot membranes
- Visualizing positive protein bands in situ
- Routine molecular biology laboratory use
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
BCIP/NBT Alkaline Phosphatase Color Development Kit FAQ
What does the kit contain?
AP reaction buffer (100 mL), NBT stock (1 mL) and BCIP stock (1 mL).
What color does it give?
A blue-purple precipitate at positive bands on the membrane.
How should the components be stored?
AP reaction buffer at room temperature or 4 °C; NBT and BCIP stocks at -20 °C away from light.
How do I order CYPP25?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Research reagents.