CYPP24 is a chemiluminescent substrate kit for detecting horseradish peroxidase (HRP)-labelled secondary antibodies in Western blot. It is based on a new-generation enhanced chemiluminescent substrate with optimized components, low background and good stability. Sensitivity is stated as tens of times higher than ordinary ECL reagent.
Catalog no. CYPP24 · For research use only
Key features
- Based on an enhanced chemiluminescent substrate; low background and good stability
- Sensitivity stated as tens of times higher than ordinary ECL
- Signal can be read on X-ray film, with a luminometer, or by CCD scanning
- Solutions A and B supplied in equal volumes
- Replacement hydrogen peroxide recipe restores sensitivity if solution B ages; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPP2402 | UltraECL Western Blot Detection Kit | 100 mL (50 mL A + 50 mL B) |
| CYPP2403 | UltraECL Western Blot Detection Kit | 500 mL (250 mL A + 250 mL B) |
Specifications
| Format | Two-part chemiluminescent substrate (A and B) |
|---|---|
| Enzyme | Horseradish peroxidase (HRP) |
| Detection | X-ray film, luminometer, CCD |
| Supply | 100 mL (50 mL + 50 mL) or 500 mL (250 mL + 250 mL) |
| Use | Research use only |
Applications
- Chemiluminescent detection of Western blots with HRP-labelled secondary antibody
- X-ray film exposure
- CCD or luminometer imaging
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
UltraECL Western Blot Detection Kit FAQ
What if solution B loses sensitivity over time?
Hydrogen peroxide in solution B can decompose. Make fresh H2O2 solution by adding 40 μL of 30% H2O2 to every 10 mL of pure water, and use it in place of solution B to restore the maximum sensitivity.
How can I read the signal?
Expose X-ray film, read with a luminometer, or scan with a CCD imager.
What secondary antibody does it need?
An HRP-labelled secondary antibody.
How do I order CYPP24?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Research reagents.