CYPP11 is a universal protein extraction reagent: a Tris buffer system (20 mM Tris pH 7.5, EDTA, NaCl) with a special nonionic detergent and phosphatase inhibitors such as sodium pyrophosphate. It lyses cells under non-denaturing conditions to release cytoplasmic proteins, soluble membrane proteins and nuclear proteins while keeping protein interactions, antigen-antibody binding and enzyme activity.
Catalog no. CYPP11 · For research use only
Key features
- Non-denaturing lysis releasing cytoplasmic, soluble membrane and nuclear proteins
- Preserves protein-protein interactions and function, including antigen-antibody binding and enzymatic activity
- Several phosphatase inhibitors, suited to phosphorylated protein co-IP and Western blot
- Suited to antibodies that recognize native conformation or non-denatured binding sites
- Add protease inhibitors such as PMSF before use; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPP1101 | Universal Protein Extraction Reagent | 50 mL |
| CYPP1102 | Universal Protein Extraction Reagent | 100 mL |
Specifications
| Format | Lysis reagent |
|---|---|
| Buffer | 20 mM Tris pH 7.5, EDTA, NaCl, nonionic detergent |
| Inhibitors | Phosphatase inhibitors incl. sodium pyrophosphate |
| Add before use | Protease inhibitors (PMSF, leupeptin, aprotinin) |
| Quantification | BCA assay (CYPP01); not suitable for Bradford |
| Working temperature | On ice or 4 °C |
| Use | Research use only |
Applications
- Western blot and immunoprecipitation
- Co-immunoprecipitation of interacting proteins
- Protein activity and function assays
- Phosphorylated protein studies
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Universal Protein Extraction Reagent FAQ
Does it denature protein?
No. It lyses under non-denaturing conditions and keeps protein properties and function.
What must I add?
Protease inhibitors such as PMSF, and optionally leupeptin, aprotinin or others. Perform all steps on ice or at 4 °C.
How do I measure protein concentration?
Use the BCA kit (CYPP01); the detergent concentration makes it unsuitable for the Bradford method.
How do I order CYPP11?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Research reagents.